Evidence map›Paper›PMID 40360917›Full record

ReviewNature methods2025

Illuminating life processes by vibrational probes.

Naixin Qian, Zhilun Zhao, Elsy El Khoury, Xin Gao, Carli Canela, Yihui Shen, Lingyan Shi, Lixue Shi, Fanghao Hu, Lu Wei and 1 more

Abstract readReview
In one paragraph

Review in Nature methods, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed.

  1. Review
  2. Review
  3. Article
  4. Article
  5. Article
  6. Vibrational Probes in Bioimaging and Chemical Biology.Chemical & biomedical imaging · 2025
    Article
  7. Bond-Selective Imaging at the Frontier of Biomedicine.Chemical & biomedical imaging · 2025
    Article
  8. Review
  9. Article
  10. Review
  11. Review
  12. Article
  13. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Naixin QianDepartment of Chemistry, Columbia University, New York, NY, USA.
Zhilun ZhaoDepartment of Chemistry, Columbia University, New York, NY, USA.
Elsy El KhouryDepartment of Chemistry, Columbia University, New York, NY, USA.ORCID http://orcid.org/0000-0002-9414-7032
Xin GaoDepartment of Chemistry, Columbia University, New York, NY, USA.ORCID http://orcid.org/0000-0002-0911-3656
Carli CanelaDepartment of Chemistry, Columbia University, New York, NY, USA.
Yihui ShenDepartment of Bioengineering, University of Pennsylvania, Philadelphia, PA, USA.
Lingyan ShiShu Chien-Gene Lay Department of Bioengineering, University of California San Diego, La Jolla, CA, USA.ORCID http://orcid.org/0000-0003-1373-3206
Lixue ShiShanghai Xuhui Central Hospital, Zhongshan-Xuhui Hospital, and Shanghai Key Laboratory of Medical Epigenetics, International Co-laboratory of Medical Epigenetics and Metabolism, Institutes of Biomedical Sciences, Shanghai Medical College, Fudan University, Shanghai, China.ORCID http://orcid.org/0000-0001-9712-5800
Fanghao HuDepartment of Chemistry, MOE Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology, Tsinghua University, Beijing, China.ORCID http://orcid.org/0000-0002-8659-4027
Lu WeiDivision of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA, USA.ORCID http://orcid.org/0000-0001-9170-2283
Wei MinDepartment of Chemistry, Columbia University, New York, NY, USA. wm2256@columbia.edu.ORCID http://orcid.org/0000-0003-2570-3557

Funding

Super-multiplex optical imaging: development of novel spectroscopy and probes to illuminate complex biomedicineR35GM149256 · NIGMS · COLUMBIA UNIV NEW YORK MORNINGSIDE · PI Wei Min · 2023 to 2026
$2.8M
Sugar Probed SRS Volumetric imaging of Metabolic ActivitiesR01GM149976 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Lingyan Shi · 2023 to 2026
$1.6M
Novel Optical Imaging Approach to Study Neurovascular Coupling SystemR21NS125395 · NINDS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI SHI, LINGYAN · 2022 to 2022
$435k
NIGMS NIH HHS R01 GM149976NIGMS NIH HHS R35 GM149256NINDS NIH HHS R21 NS125395
6 · The paper itself

Abstract

Vibration of chemical bonds can serve as imaging contrast. Vibrational probes, synergized with major advances in chemical bond imaging instruments, have recently flourished and proven valuable in illuminating life processes. Here, we review how the development of vibrational probes with optimal biocompatibility, enhanced sensitivity, multichromatic colors and diverse functionality has extended chemical bond imaging beyond the prevalent label-free paradigm into various novel applications such as imaging metabolites, metabolic imaging, drug imaging, super-multiplex imaging, vibrational profiling and vibrational sensing. These advancements in vibrational probes have greatly facilitated understanding living systems, a new field of vibrational chemical biology.

Indexed as

Molecular ImagingMolecular ProbesVibrationAnimalsHumansMolecular Probes

Identifiers

PMID40360917
PMCPMC12626105

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.