Evidence map›Paper›PMID 40360693›Full record

ArticleCommunications biology2025

Unveiling the impact of cGMP-dependent protein kinase of Neospora caninum on calcium fluxes and egress functions through quantitative phosphoproteome analysis.

Xianmei Wang, Kun Guo, Zhili Shan, Zhu Ying, Zifu Zhu, Shiman Yang, Na Yang, Qun Liu, Lifang Wang, Jing Liu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Communications biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Xianmei WangNational Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing, PR China.
Kun GuoNational Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing, PR China.
Zhili ShanNational Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing, PR China.
Zhu YingNational Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing, PR China.
Zifu ZhuNational Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing, PR China.
Shiman YangNational Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing, PR China.
Na YangNational Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing, PR China.
Qun LiuNational Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing, PR China.
Lifang WangNational Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing, PR China.
Jing LiuNational Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing, PR China. liujingvet@cau.edu.cn.ORCID http://orcid.org/0000-0001-7309-0340

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Neospora caninum, a pathogen associated with abortion in pregnant cattle and motor nerve disorders in dogs, poses a substantial threat. Cyclic GMP-dependent protein kinase (PKG) functions as a central signal transduction hub in apicomplexan parasites. However, PKG has not been characterized in N. caninum, and its targets and pathways controlled by PKG remain unknown. Using a mini auxin-inducible degron system (mAID), we knocked down PKG in N. caninum, demonstrating its indispensable role in tachyzoite invasion and egress from host cells. PKG promotes microneme secretion and enhances tachyzoite gliding motility by elevating intracellular Ca

Indexed as

CalciumCyclic GMP-Dependent Protein KinasesNeosporaPhosphoproteinsProteomeProtozoan ProteinsAnimalsPhosphorylationProteomicsCalciumCyclic GMP-Dependent Protein KinasesPhosphoproteinsProteomeProtozoan Proteins

Identifiers

PMID40360693
PMCPMC12075863

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.