Evidence map›Paper›PMID 40360526›Full record

ArticleNPJ biofilms and microbiomes2025

SadB, a mediator of AmrZ proteolysis and biofilm development in Pseudomonas aeruginosa.

Yossi Ben-David, Michael Sporny, Yigal Brochin, Bar Piscon, Shira Roth, Itzhak Zander, Michal Nisani, Sivan Shoshani, Orly Yaron, Sarit Karako-Lampert and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in NPJ biofilms and microbiomes, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. UnderstandingMicroorganisms · 2026
    Review
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Yossi Ben-DavidThe Mina & Everard Goodman Faculty of Life Sciences, The Institute of Nanotechnology and Advanced Materials, Bar-Ilan University, Max and Anna Webb Street, Ramat Gan, 5290002, Israel.
Michael SpornyThe Mina & Everard Goodman Faculty of Life Sciences, The Institute of Nanotechnology and Advanced Materials, Bar-Ilan University, Max and Anna Webb Street, Ramat Gan, 5290002, Israel.
Yigal BrochinThe Mina & Everard Goodman Faculty of Life Sciences, The Institute of Nanotechnology and Advanced Materials, Bar-Ilan University, Max and Anna Webb Street, Ramat Gan, 5290002, Israel.
Bar PisconThe Mina & Everard Goodman Faculty of Life Sciences, The Institute of Nanotechnology and Advanced Materials, Bar-Ilan University, Max and Anna Webb Street, Ramat Gan, 5290002, Israel.
Shira RothThe Alexander Kofkin Faculty of Engineering, The Institute of Nanotechnology and Advanced Materials, Bar-Ilan University, Max and Anna Webb Street, Ramat Gan, 5290002, Israel.
Itzhak ZanderThe Mina & Everard Goodman Faculty of Life Sciences, The Institute of Nanotechnology and Advanced Materials, Bar-Ilan University, Max and Anna Webb Street, Ramat Gan, 5290002, Israel.
Michal NisaniThe Mina & Everard Goodman Faculty of Life Sciences, The Institute of Nanotechnology and Advanced Materials, Bar-Ilan University, Max and Anna Webb Street, Ramat Gan, 5290002, Israel.
Sivan ShoshaniThe Mina & Everard Goodman Faculty of Life Sciences, The Institute of Nanotechnology and Advanced Materials, Bar-Ilan University, Max and Anna Webb Street, Ramat Gan, 5290002, Israel.
Orly YaronThe Scientific Equipment Center, Bar-Ilan University, Max and Anna Webb Street, Ramat Gan, 5290002, Israel.
Sarit Karako-LampertThe Scientific Equipment Center, Bar-Ilan University, Max and Anna Webb Street, Ramat Gan, 5290002, Israel.
Ilana Lebenthal-LoingerThe Mina & Everard Goodman Faculty of Life Sciences, The Institute of Nanotechnology and Advanced Materials, Bar-Ilan University, Max and Anna Webb Street, Ramat Gan, 5290002, Israel.
Amos DanielliThe Alexander Kofkin Faculty of Engineering, The Institute of Nanotechnology and Advanced Materials, Bar-Ilan University, Max and Anna Webb Street, Ramat Gan, 5290002, Israel.
Yarden OpatowskyThe Mina & Everard Goodman Faculty of Life Sciences, The Institute of Nanotechnology and Advanced Materials, Bar-Ilan University, Max and Anna Webb Street, Ramat Gan, 5290002, Israel.
Ehud BaninThe Mina & Everard Goodman Faculty of Life Sciences, The Institute of Nanotechnology and Advanced Materials, Bar-Ilan University, Max and Anna Webb Street, Ramat Gan, 5290002, Israel. ehud.banin@biu.ac.il.

Funding

Israel Science Foundation 1694/22 and 1125/17
6 · The paper itself

Abstract

The ability of bacteria to commit to surface colonization and biofilm formation is a highly regulated process. In this study, we characterized the activity and structure of SadB, initially identified as a key regulator in the transition from reversible to irreversible surface attachment. Our results show that SadB acts as an adaptor protein that tightly regulates the master regulator AmrZ at the post-translational level. SadB directly binds to the C-terminal domain of AmrZ, leading to its rapid degradation, primarily by the Lon protease. Structural analysis suggests that SadB does not directly interact with small molecules upon signal transduction, differing from previous findings in Pseudomonas fluorescens. Instead, the SadB structure supports its role in mediating protein-protein interactions, establishing it as a major checkpoint for biofilm commitment.

Indexed as

Bacterial ProteinsBiofilmsPseudomonas aeruginosaGene Expression Regulation, BacterialProtein BindingProteolysisBacterial Proteins

Identifiers

PMID40360526
PMCPMC12075610

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.