Evidence map›Paper›PMID 40359924›Full record

ArticleThe American journal of tropical medicine and hygiene2025

Differential Virulence and Host-Specific Fitness of Regionally Distinct Human-Derived Powassan Virus Lineage 2 Strains.

Rachel E Lange, Julia C Pritchard, Anna S Jaeger, Tyler D Bold, Jennifer L White, Amy B Dean, Rene C Hull, Kirsten St George, Alan P Dupuis, Matthew T Aliota and 1 more

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In one paragraph

Article in The American journal of tropical medicine and hygiene, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Rachel E LangeArbovirus Laboratory, Wadsworth Center, New York State Department of Health, Slingerlands, New York.
Julia C PritchardDepartment of Veterinary and Biomedical Sciences, University of Minnesota Twin Cities, St. Paul, Minnesota.
Anna S JaegerDepartment of Veterinary and Biomedical Sciences, University of Minnesota Twin Cities, St. Paul, Minnesota.
Tyler D BoldDivision of Infectious Diseases and International Medicine, Department of Medicine, Center for Immunology, University of Minnesota Medical School, Minneapolis, Minnesota.
Jennifer L WhiteBureau of Communicable Disease Control, New York State Department of Health, Albany, New York.
Amy B DeanLaboratory of Viral Diseases, New York State Department of Health, Albany, New York.
Rene C HullLaboratory of Viral Diseases, New York State Department of Health, Albany, New York.
Kirsten St GeorgeDepartment of Biomedical Sciences, State University of New York University at Albany School of Public Health, Albany, New York.
Alan P DupuisArbovirus Laboratory, Wadsworth Center, New York State Department of Health, Slingerlands, New York.
Matthew T AliotaDepartment of Veterinary and Biomedical Sciences, University of Minnesota Twin Cities, St. Paul, Minnesota.
Alexander T CiotaArbovirus Laboratory, Wadsworth Center, New York State Department of Health, Slingerlands, New York.

Funding

Powassan virus evolution, fitness, and pathogenesis in the Northeast and Midwest United StatesR01AI179758 · NIAID · UNIVERSITY OF MINNESOTA · PI Matthew T Aliota, Alexander Timothy Ciota · 2024 to 2026
$2.1M
NIAID NIH HHS R01 AI179758
6 · The paper itself

Abstract

Powassan virus (POWV; family Flaviviridae) is a tick-borne encephalitic virus endemic to Canada, the United States, and Russia. In the United States, POWV is transmitted by ixodid ticks, and transmission foci reflect the geographic range of these vectors, primarily Ixodes scapularis. Thus, northeastern and midwestern regions of the United States contain the highest human case burdens and prevalence of infected ticks. Notably, New York (NY) and Minnesota (MN) have a long history of POWV transmission to humans. Over time, genetic divergence has occurred in these regions, giving rise to distinct midwestern and northeastern clades. Despite the established circulation of POWV, increases in reported human cases, and documented genetic distinction, an understanding of strain-specific POWV virulence is limited because of the lack of human isolates. In 2020 and 2021, two POWV strains were isolated from fatal human cases from MN (deer tick virus [DTV] MN-PV320) and NY (DTV NY21-027). Here, we provide the first characterization of geographically distinct, contemporary, human POWV isolates. Comprehensive genetic characterization was completed and phenotypic variability was determined in vitro and in vivo . Although strain fitness was similar in I. scapularis, higher mortality rates were measured in a susceptible POWV mouse model after infection with DTV NY21-027 compared with DTV MN-PV320. Genetic analysis revealed several variable amino acid substitutions, including I2173L in DTV NY21-027, which was selected for in all strains after neurological infection. These data suggest that genetic divergence of POWV strains from regionally distinct transmission foci could contribute to strain-dependent pathogenic potential in humans.

Indexed as

Encephalitis, Tick-BorneEncephalitis Viruses, Tick-BorneAnimalsHost SpecificityHumansIxodesMicePhylogenyVirulence

Identifiers

PMID40359924
PMCPMC12225551

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.