Evidence map›Paper›PMID 40359194›Full record

ArticlePLoS neglected tropical diseases2025

Clonorchis sinensis extracellular vesicles associated with Csi-let-7a-5p activate pro-inflammatory macrophages to induce biliary injury.

Beibei Zhang, Xing Li, Qian-Yang Zhou, Chen Zhang, Zheng-Rui Bian, Xin-Xin Ren, Qian Yu, Hui Hua, Zhihua Jiang, Bo Zhang and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PLoS neglected tropical diseases, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
  2. The Liver FlukeBiomolecules · 2026
    Article
  3. Article
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Beibei ZhangJiangsu Key Laboratory of Immunity and Metabolism, Jiangsu International Key Laboratory of Immunity and Metabolism, National Demonstration Center for Experimental Basic Medical Science Education, Department of Pathogenic Biology and Immunology, School of Basic Medical Science, Xuzhou Medical University, Xuzhou, People's Republic of China.
Xing LiJiangsu Key Laboratory of Immunity and Metabolism, Jiangsu International Key Laboratory of Immunity and Metabolism, National Demonstration Center for Experimental Basic Medical Science Education, Department of Pathogenic Biology and Immunology, School of Basic Medical Science, Xuzhou Medical University, Xuzhou, People's Republic of China.
Qian-Yang ZhouJiangsu Key Laboratory of Immunity and Metabolism, Jiangsu International Key Laboratory of Immunity and Metabolism, National Demonstration Center for Experimental Basic Medical Science Education, Department of Pathogenic Biology and Immunology, School of Basic Medical Science, Xuzhou Medical University, Xuzhou, People's Republic of China.
Chen ZhangJiangsu Key Laboratory of Immunity and Metabolism, Jiangsu International Key Laboratory of Immunity and Metabolism, National Demonstration Center for Experimental Basic Medical Science Education, Department of Pathogenic Biology and Immunology, School of Basic Medical Science, Xuzhou Medical University, Xuzhou, People's Republic of China.
Zheng-Rui BianJiangsu Key Laboratory of Immunity and Metabolism, Jiangsu International Key Laboratory of Immunity and Metabolism, National Demonstration Center for Experimental Basic Medical Science Education, Department of Pathogenic Biology and Immunology, School of Basic Medical Science, Xuzhou Medical University, Xuzhou, People's Republic of China.
Xin-Xin RenJiangsu Key Laboratory of Immunity and Metabolism, Jiangsu International Key Laboratory of Immunity and Metabolism, National Demonstration Center for Experimental Basic Medical Science Education, Department of Pathogenic Biology and Immunology, School of Basic Medical Science, Xuzhou Medical University, Xuzhou, People's Republic of China.
Qian YuJiangsu Key Laboratory of Immunity and Metabolism, Jiangsu International Key Laboratory of Immunity and Metabolism, National Demonstration Center for Experimental Basic Medical Science Education, Department of Pathogenic Biology and Immunology, School of Basic Medical Science, Xuzhou Medical University, Xuzhou, People's Republic of China.
Hui HuaJiangsu Key Laboratory of Immunity and Metabolism, Jiangsu International Key Laboratory of Immunity and Metabolism, National Demonstration Center for Experimental Basic Medical Science Education, Department of Pathogenic Biology and Immunology, School of Basic Medical Science, Xuzhou Medical University, Xuzhou, People's Republic of China.
Zhihua JiangInstitute of Parasitic Disease Control and Prevention, Guangxi Key Laboratory for the Prevention and Control of Viral Hepatitis, Guangxi Zhuang Autonomous Region Center for Disease Control and Prevention, Nanning, China.
Bo ZhangJiangsu Key Laboratory of Immunity and Metabolism, Jiangsu International Key Laboratory of Immunity and Metabolism, National Demonstration Center for Experimental Basic Medical Science Education, Department of Pathogenic Biology and Immunology, School of Basic Medical Science, Xuzhou Medical University, Xuzhou, People's Republic of China.
Xiang-Yang LiJiangsu Key Laboratory of Immunity and Metabolism, Jiangsu International Key Laboratory of Immunity and Metabolism, National Demonstration Center for Experimental Basic Medical Science Education, Department of Pathogenic Biology and Immunology, School of Basic Medical Science, Xuzhou Medical University, Xuzhou, People's Republic of China.
Mu-Xin ChenNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research), NHC Key Laboratory of Parasite and Vector Biology, Shanghai, People's Republic of China.
Kui-Yang ZhengJiangsu Key Laboratory of Immunity and Metabolism, Jiangsu International Key Laboratory of Immunity and Metabolism, National Demonstration Center for Experimental Basic Medical Science Education, Department of Pathogenic Biology and Immunology, School of Basic Medical Science, Xuzhou Medical University, Xuzhou, People's Republic of China.
Chao YanJiangsu Key Laboratory of Immunity and Metabolism, Jiangsu International Key Laboratory of Immunity and Metabolism, National Demonstration Center for Experimental Basic Medical Science Education, Department of Pathogenic Biology and Immunology, School of Basic Medical Science, Xuzhou Medical University, Xuzhou, People's Republic of China.ORCID 0000-0002-7482-9116

Funding

Independent Research Projects on the Open Sharing of Large Scientific Research Instruments in Jiangsu ProvinceKey Research and Development Program in Hainan ProvinceNational Natural Science Foundation of Guangxi Zhuang Autonomous RegionNatural Science Foundation of ChinaThree-Year Public Health Action Plan (2023-2025) of Shanghai
6 · The paper itself

Abstract

During Clonorchis sinensis (C. sinensis) infection, pro-inflammatory macrophages (M1 macrophages) are highly activated, yet their role in the disease remains poorly understood. Previous studies have demonstrated that extracellular vesicles from C. sinensis (CsEVs) can activate these macrophages, and inhibiting a specific miRNA (Csi-let-7a-5p) in CsEVs (InCsEVs) can reduce this activation. In the present study, liver macrophages in mice were removed using clodronate liposomes (Clodlip). Subsequently, different types of bone marrow-derived macrophages (BMDMs) were adoptively transferred into the mice lacking liver macrophages: untreated (PBS-BMDM), treated with CsEVs (CsEVs-BMDM), treated with a control (ScrCsEVs-BMDM), or treated with InCsEVs (InCsEVs-BMDM). Biliary damages were then evaluated. The results indicated that the transferred macrophages successfully repopulated the mice. CsEVs-BMDM led to significant inflammation and bile duct damage, accompanied by higher levels of inflammatory cytokines (TNF-α and IL-1β). However, when macrophages were treated with InCsEVs, the damage and inflammation were alleviated, and the levels of TNF-α and IL-1β decreased. These findings suggest that pro-inflammatory macrophages activated by CsEVs, especially through Csi-let-7a-5p, play a crucial role in biliary damage during C. sinensis infection. Although other immune cells may also be involved, this study emphasizes the significance of pro-inflammatory macrophages in clonorchiasis.

Indexed as

ClonorchiasisClonorchis sinensisExtracellular VesiclesMacrophagesMicroRNAsAnimalsCytokinesLiverMaleMiceMice, Inbred C57BLCytokinesMicroRNAsmirnlet7 microRNA, mouse

Identifiers

PMID40359194
PMCPMC12074333

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.