ArticleeLife2025
Dual-color optical activation and suppression of neurons with high temporal precision.
Article in eLife, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Single-Die-Level MEMS Post-Processing for Prototyping CMOS-Based Neural Probes Combined with Optical Fibers for Optogenetic Neuromodulation.Micromachines · 2026Article
- Strategies to Decipher Neuron Identity from Extracellular Recordings in Behaving Nonhuman Primates.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2025Article
- A simplified two-plasmid system for orthogonal control of mammalian gene expression using light-activated CRISPR effector.BMC biotechnology · 2025Article
- Article
- Article
- Practical considerations in an era of multicolor optogenetics.Frontiers in cellular neuroscience · 2023Article
- Red Light Optogenetics in Neuroscience.Frontiers in cellular neuroscience · 2021Review
Corrections and comments
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Authors and funding
12 authors.
Funding
Abstract
A well-known phenomenon in the optogenetic toolbox is that all light-gated ion channels, including red-shifted channelrhodopsins (ChRs), are activated by blue light, whereas blue-shifted ChRs are minimally responsive to longer wavelengths. Here, we took advantage of this feature to create a system which allows high-frequency activation of neurons with pulses of red light, while permitting the suppression of action potentials (APs) with millisecond precision by blue light. We achieved this by pairing an ultrafast red-shifted ChR with a blue light-sensitive anion channel of appropriately matching kinetics. This required screening several anion-selective ChRs, followed by a model-based mutagenesis strategy to optimize their kinetics and light spectra. Slice electrophysiology in the hippocampus as well as behavioral inspection of vibrissa movement demonstrate a minimal excitation from blue light. Of significant potential value, in contrast to existing tools, the system we introduce here allows high-frequency optogenetic excitation of neurons with red light, while blue light suppression of APs is confined within the duration of the light pulse.
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Registered trials
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