ArticleeLife2025
Reproducibility of in vivo electrophysiological measurements in mice.
Article in eLife, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed.
- Article
- Age-related changes in behavioural and neural variability in a decision-making task.Nature communications · 2026Article
- Beast3D: Animal behavioral analysis and neural encoding from multi-view video via Gaussian splatting.ArXiv · 2026Article
- SpikeLab: Agentic tools for spike data analysis.bioRxiv : the preprint server for biology · 2026Article
- Lightning Pose 3D: an uncertainty-aware framework for data-efficient multi-view animal pose estimation.bioRxiv : the preprint server for biology · 2026Article
- A multimodal approach for visualizing and identifying electrophysiological cell types in vivo.Nature communications · 2026Article
- A flexible quality metric for electrophysiological recordings across brain regions and species.bioRxiv : the preprint server for biology · 2026Article
- Animal behavioral analysis and neural encoding with transformer-based self-supervised pretraining.ArXiv · 2026Article
- Exploiting correlations across trials and behavioral sessions to improve neural decoding.Neuron · 2026Article
- How distributed is the brain-wide network that is recruited for cognition?Nature reviews. Neuroscience · 2026Review
- Locomotion selectively enhances visual speed encoding in mouse medial higher visual areas.iScience · 2026Article
- Ultra-high-density Neuropixels probes improve detection and identification in neuronal recordings.Neuron · 2025Article
- Identifying the factors governing internal state switches during nonstationary sensory decision-making.Nature communications · 2025Article
- VTA dopamine neuron activity produces spatially organized value representations.bioRxiv : the preprint server for biology · 2025Article
- Inpainting the Neural Picture: Inferring Unrecorded Brain Area Dynamics from Multi-Animal Datasets.ArXiv · 2025Article
- Exploiting correlations across trials and behavioral sessions to improve neural decoding.bioRxiv : the preprint server for biology · 2025Article
- Decoding decision-making behavior from sparse neural spiking activity.PLoS computational biology · 2025Article
- Striatal pathways oppositely shift cortical activity along the decision axis.bioRxiv : the preprint server for biology · 2025Article
- A common computational and neural anomaly across mouse models of autism.Nature neuroscience · 2025Article
- Article
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Authors and funding
46 authors.
Funding
Abstract
Understanding brain function relies on the collective work of many labs generating reproducible results. However, reproducibility has not been systematically assessed within the context of electrophysiological recordings during cognitive behaviors. To address this, we formed a multi-lab collaboration using a shared, open-source behavioral task and experimental apparatus. Experimenters in 10 laboratories repeatedly targeted Neuropixels probes to the same location (spanning secondary visual areas, hippocampus, and thalamus) in mice making decisions; this generated a total of 121 experimental replicates, a unique dataset for evaluating reproducibility of electrophysiology experiments. Despite standardizing both behavioral and electrophysiological procedures, some experimental outcomes were highly variable. A closer analysis uncovered that variability in electrode targeting hindered reproducibility, as did the limited statistical power of some routinely used electrophysiological analyses, such as single-neuron tests of modulation by individual task parameters. Reproducibility was enhanced by histological and electrophysiological quality-control criteria. Our observations suggest that data from systems neuroscience is vulnerable to a lack of reproducibility, but that across-lab standardization, including metrics we propose, can serve to mitigate this.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.