Evidence map›Paper›PMID 40354066›Full record

ArticleTranslational vision science & technology2025

A Simple and Rapid Method for Simultaneous Isolation of Mouse Retina and RPE Wholemounts.

Jialiang Yang, Haiping Wu, Qi Li, Jiaxin Guo, Jialin Yao, Shengliu Pan, Xiawei Wu, Haotian Huang, Runfang Chen, Junnian Chen and 4 more

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Article in Translational vision science & technology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Jialiang YangSichuan Provincial Key Laboratory for Human Disease Gene Study, Department of Laboratory Medicines, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, China.
Haiping WuSichuan Provincial Key Laboratory for Human Disease Gene Study, Department of Laboratory Medicines, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, China.
Qi LiSichuan Provincial Key Laboratory for Human Disease Gene Study, Department of Laboratory Medicines, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, China.
Jiaxin GuoSichuan Provincial Key Laboratory for Human Disease Gene Study, Department of Laboratory Medicines, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, China.
Jialin YaoSichuan Provincial Key Laboratory for Human Disease Gene Study, Department of Laboratory Medicines, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, China.
Shengliu PanCenter for Natural Products Research, Chengdu Institute of Biology, Chinese Academy of Sciences, Tianfu New District, Chengdu, China.
Xiawei WuSichuan Provincial Key Laboratory for Human Disease Gene Study, Department of Laboratory Medicines, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, China.
Haotian HuangSichuan Provincial Key Laboratory for Human Disease Gene Study, Department of Laboratory Medicines, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, China.
Runfang ChenSichuan Provincial Key Laboratory for Human Disease Gene Study, Department of Laboratory Medicines, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, China.
Junnian ChenSichuan Provincial Key Laboratory for Human Disease Gene Study, Department of Laboratory Medicines, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, China.
Yubin WangSichuan Provincial Key Laboratory for Human Disease Gene Study, Department of Laboratory Medicines, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, China.
Yue PengSichuan Provincial Key Laboratory for Human Disease Gene Study, Department of Laboratory Medicines, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, China.
Fuhua WuSichuan Provincial Key Laboratory for Human Disease Gene Study, Department of Laboratory Medicines, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, China.
Jing HuSichuan Provincial Key Laboratory for Human Disease Gene Study, Department of Laboratory Medicines, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Purpose: Retina and retinal pigment epithelium (RPE) wholemounts are important models for studying the pathophysiology of retinal-related ophthalmic diseases, such as age-related macular degeneration and diabetic retinopathy. Currently, there is no method available for simultaneously obtaining retina and RPE wholemounts. The aim of this study is to develop a simple, rapid, and effective technique for the simultaneous isolation of mouse retina and RPE wholemounts. Methods: We developed a novel, streamlined procedure for the efficient isolation of intact retina and RPE wholemounts from mouse eyes. The method involves minimal dissection and uses basic laboratory equipment, allowing the entire process to be completed in approximately 2 to 5 minutes per sample. The study also explores the impact of different fixation times on the structural integrity and quality of both retina and RPE wholemounts (3 hours in 4% paraformaldehyde [PFA], 30 minutes < 1 × phosphate-buffered saline [PBS] < 3 hours). Results: The new method consistently yields high-quality, intact retina and RPE wholemounts, with excellent structural integrity suitable for downstream imaging and molecular analyses. The technique significantly reduces preparation time. Optimal fixation conditions were identified, with 3 hours of fixation in 4% PFA and PBS incubation times between 30 minutes and 3 hours yielding the best results. The approach showed higher tissue integrity (80% vs. 45%) and improved staining quality of photoreceptor and ganglion cells. Additionally, the method is highly reproducible and effective for wholemount preparations from both young and older mice (6 and 12 months). Conclusions: This study presents a significant advancement in the preparation of retina and RPE wholemounts. The simplicity, speed, and preservation of tissue integrity of the new method make it a valuable tool for ophthalmic disease research. Its potential applications include drug screening, gene therapy, and disease modeling, offering significant advantages in time efficiency, reproducibility, and the quality of morphological analysis.

Indexed as

RetinaRetinal Pigment EpitheliumAnimalsMiceMice, Inbred C57BLTissue Fixation

Identifiers

PMID40354066
PMCPMC12080734

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.