Evidence map›Paper›PMID 40352643›Full record

ArticlePNAS nexus2025

The priming phosphorylation of KaiC is activated by the release of its autokinase autoinhibition.

Yoshihiko Furuike, Yasuhiro Onoue, Shinji Saito, Toshifumi Mori, Shuji Akiyama

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PNAS nexus, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Yoshihiko FuruikeResearch Center of Integrative Molecular Systems (CIMoS), Institute for Molecular Science, National Institutes of Natural Sciences, 38 Nishigo-Naka, Myodaiji, Okazaki 444-8585, Japan.ORCID https://orcid.org/0000-0002-4901-7394
Yasuhiro OnoueResearch Center of Integrative Molecular Systems (CIMoS), Institute for Molecular Science, National Institutes of Natural Sciences, 38 Nishigo-Naka, Myodaiji, Okazaki 444-8585, Japan.ORCID https://orcid.org/0000-0002-3152-0047
Shinji SaitoMolecular Science Program, Graduate Institute for Advanced Studies, SOKENDAI, 38 Nishigo-Naka, Myodaiji, Okazaki 444-8585, Japan.ORCID https://orcid.org/0000-0003-4982-4820
Toshifumi MoriDivision of Applied Molecular Chemistry, Institute for Materials Chemistry and Engineering, Kyushu University, 6-1 Kasugakoen, Kasuga, Fukuoka 816-8580, Japan.ORCID https://orcid.org/0000-0003-0188-0794
Shuji AkiyamaResearch Center of Integrative Molecular Systems (CIMoS), Institute for Molecular Science, National Institutes of Natural Sciences, 38 Nishigo-Naka, Myodaiji, Okazaki 444-8585, Japan.ORCID https://orcid.org/0000-0002-7184-189X

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

KaiC, a cyanobacterial circadian clock protein with autokinase activity, catalyzes the dual phosphorylation of its own S431 and T432 residues in a circadian manner in the presence of KaiA and KaiB. Priming phosphorylation at T432 is a key step that promotes secondary phosphorylation at S431. Although KaiA binding is considered essential for KaiC phosphorylation, the mechanisms underlying the activation and inactivation of priming phosphorylation remain elusive. We found that although the priming phosphorylation is autoinhibited within KaiC, it actually proceeds at a rate constant of 0.019 h

Indexed as

circadian clockcyanobacteriaKaiCkinasepriming phosphorylation

Identifiers

PMID40352643
PMCPMC12065004

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.