ArticleBiomarkers in medicine2025
PCR targeting 16S rRNA,
Article in Biomarkers in medicine, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
aimsPolymerase chain reaction (PCR) is a rapid molecular technique for pathogen detection, but its application in detecting group B Streptococcus (GBS) requires further exploration. This study assessed the diagnostic performance of the PCR method for GBS detection in pregnant women with 35 to 37 weeks of pregnancy.
methodsThis study enrolled 116 women at 35-37 weeks of pregnancy and simultaneously collected two vaginal swab samples for GBS detection via culture and PCR methods.
resultsThrough the culture method, 21 (18.1%) samples were positive for GBS. Moreover, 24 (20.7%), 23 (19.8%), and 23 (19.8%) samples were positive for GBS according to PCR methods targeting 16S rRNA, cfb, and hylB, respectively. The results of PCR-based GBS detection of 16S rRNA (Kappa value = 0.917,
conclusionPCR targeting 16S rRNA,
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