Evidence map›Paper›PMID 40350417›Full record

ArticleMalaria journal2025

Assessment of the in vitro activity and selectivity of Artemisia afra and Artemisia annua aqueous extracts against artemisinin-resistant Plasmodium falciparum.

Camille Roesch, Kutub Ashraf, Amélie Vantaux, Adriana A Marin, Steven P Maher, Jean-Francois Franetich, Nimol Kloeung, Sopheakvatey Ke, Hoa Thi My Vo, Jean-Christophe Barale and 2 more

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In one paragraph

Article in Malaria journal, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Phytochemical Composition of Clonally PropagatedMolecules (Basel, Switzerland) · 2026
    Article
  2. Immunomodulatory Effect ofAnimals : an open access journal from MDPI · 2025
    Article
  3. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

12 authors.

Camille Roesch *Malaria Molecular Epidemiology Unit, Institut Pasteur du Cambodge, Phnom Penh, Cambodia. roesch.camille@gmail.com.
Kutub Ashraf *INSERM, CNRS, Centre d'Immunologie et des Maladies Infectieuses, CIMI, Sorbonne Université, Paris, France.
Amélie VantauxMalaria Molecular Epidemiology Unit, Institut Pasteur du Cambodge, Phnom Penh, Cambodia.
Adriana A MarinCenter for Tropical and Emerging Global Diseases, University of Georgia, Athens, GA, USA.
Steven P MaherCenter for Tropical and Emerging Global Diseases, University of Georgia, Athens, GA, USA.
Jean-Francois FranetichINSERM, CNRS, Centre d'Immunologie et des Maladies Infectieuses, CIMI, Sorbonne Université, Paris, France.
Nimol KloeungMalaria Molecular Epidemiology Unit, Institut Pasteur du Cambodge, Phnom Penh, Cambodia.
Sopheakvatey KeMalaria Molecular Epidemiology Unit, Institut Pasteur du Cambodge, Phnom Penh, Cambodia.
Hoa Thi My VoImmunology Unit, Institut Pasteur International Network, Institut Pasteur du Cambodge, Phnom Penh, Cambodia.
Jean-Christophe BaraleInstitut Pasteur, Université Paris Cité, CNRS UMR3528, Unité de Microbiologie Structurale, Paris, France.
Dominique MazierINSERM, CNRS, Centre d'Immunologie et des Maladies Infectieuses, CIMI, Sorbonne Université, Paris, France.
Benoit WitkowskiMalaria Molecular Epidemiology Unit, Institut Pasteur du Cambodge, Phnom Penh, Cambodia.

Funding

Labex ParaFrap-IRD PhD South program ANR-11-LABEX-0024
6 · The paper itself

Abstract

backgroundThe recent emergence of artemisinin resistance in Africa is drawing scrutiny toward the use of alternative anti-malarial therapy based on Artemisia annua and Artemisia afra phytotherapies. This study aimed to determine if either A. annua and A. afra extracts are active against artemisinin-resistant Plasmodium falciparum isolates and determine the selectivity of inhibitory phytotherapies.

methodsArtemisia extracts were tested in vitro to mimic parasites exposure to extracts in population drinking Artemisia sp. teas. Artemisia extracts were tested in Ring Stage Survival Assays (RSA

resultsThe study revealed a substantially decreased in vitro activity of A. annua extracts when tested on artemisinin-resistant parasites mutated in the Pfkelch13 gene (RSA

conclusionsArtemisia annua activity is driven by artemisinin, implicating the same resistance profiles and concerns associated with semisynthetic artemisinin derivatives. Artemisia afra showed artemisinin-independent antiplasmodial activity. However, the molecular basis of this activity is unknown and may not present a sufficient selectivity, thus further characterization of A. afra is essential.

Indexed as

AntimalarialsArtemisiaArtemisia annuaArtemisininsDrug ResistancePlant ExtractsPlasmodium falciparumCambodiaHumansParasitic Sensitivity TestsAntimalarialsartemisininArtemisininsPlant ExtractsAntimalarial activityArtemisia sp.Plasmodium falciparumResistance

Identifiers

PMID40350417
PMCPMC12067737

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.