ArticleThe Journal of reproduction and development2025
In vitro embryo production via ovum pick-up (OPU) and intracytoplasmic sperm injection (ICSI) in pure and crossbred Japanese Hokkaido native ponies.
Article in The Journal of reproduction and development, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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Who cites it
1 citing paper in PubMed.
- Dynamics of plasma anti-Müllerian hormone concentrations before and after ovum pick-up in pure and crossbred Hokkaido native ponies.Journal of equine science · 2026Article
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11 authors.
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Abstract
This study evaluated the viability of in vitro embryo production using ovum pick-up (OPU) and intracytoplasmic sperm injection (ICSI) as breeding techniques for pure and crossbred Hokkaido native ponies (n = 9). Oocytes were collected using transvaginal ultrasound-guided follicle aspiration. ICSI was performed on in vitro matured oocytes using frozen semen. Embryonic cultures were monitored using time-lapse cinematography. Blastocysts were cryopreserved and, after thawing, were transferred non-surgically into recipient mares. Over nine OPU sessions, the mean number of aspirated follicles was 23.9 (range, 13-49). The oocyte recovery and maturation rates were 35.3% (76/215) and 61.5% (40/65), respectively. The cleavage rate was 57.5% (23/40). Of cleaved embryos, 56.5% (13/23) were arrested at the 4-cell to 8-cell stage, and five developed into early-blastocyst. Three embryos were transferred, resulting in a successful pregnancy. In conclusion, OPU-ICSI is a viable assisted reproductive technology for enhancing the population of Japanese native horses.
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