Evidence map›Paper›PMID 40350284›Full record

ArticleMicrobes and environments2025

Group-specific Quantification of mcrA genes of Methanogenic Archaea and "Candidatus Methanoperedens" by Digital PCR.

Takeshi Watanabe, Atsuya Endo, Rio Hamada, Rina Shinjo, Susumu Asakawa

Abstract read
In one paragraph

Article in Microbes and environments, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Takeshi WatanabeGraduate School of Bioagricultural Sciences, Nagoya University.
Atsuya EndoGraduate School of Bioagricultural Sciences, Nagoya University.
Rio HamadaSchool of Agricultural Sciences, Nagoya University.
Rina ShinjoGraduate School of Bioagricultural Sciences, Nagoya University.
Susumu AsakawaGraduate School of Bioagricultural Sciences, Nagoya University.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Digital PCR is a technique that quantifies target genes based on the absence or presence of the targets in PCR amplicons. The present study exami-ned group-specific probes for the quantification of mcrA genes in six methanogenic archaeal groups and "Candidatus Methanoperedens" by digital PCR with the universal primers ML-f and ML-r. A digital PCR ana-lysis of paddy field soil detected all the targets, with the dominant and minor groups being Methanomicrobiales and Methanobrevibacter spp., respectively (10

Indexed as

ArchaeaArchaeal ProteinsOxidoreductasesPolymerase Chain ReactionDNA, ArchaealDNA PrimersDNA Restriction EnzymesMethaneSoil MicrobiologyArchaeal ProteinsDNA, ArchaealDNA PrimersDNA Restriction Enzymesendodeoxyribonuclease McrAMethaneOxidoreductasesdigital PCRmcrA genesmethanogenic archaeapaddy field soil

Identifiers

PMID40350284
PMCPMC12213062

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.