ArticleJournal of endodontics2025
An In Vitro Tube Model for Cell Biocompatibility Study of Capping Materials for Regenerative Endodontics.
Article in Journal of endodontics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
3 citing papers in PubMed.
- Cell homing within endodontic hydrogels: A scoping review.The Japanese dental science review · 2026Review
- Dental Glass Ionomer Cement for Root Perforation Management: Physicochemical Characteristics and In Vitro Cell Response.Dentistry journal · 2026Article
- Functionalization of Photocrosslinkable GelMA Hydrogel With Metal Oxides for Direct Pulp Capping.BioMed research international · 2026Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
introductionCell-based pulp regeneration utilizes capping materials underneath a final restoration to seal the tooth after delivering cells into the canal system. Studying the immediate biocompatibility of materials with injected cells has been challenging. This study aimed to utilize a tube model to observe cell response directly and conveniently to the capping materials in a cell culture in vitro system.
methodsA tapered plastic tube (14 mm in length, 2 mm diameter of the apex opening) to simulate root canal space was filled with fluorescence-labeled dental pulp cells mixed in fibrin gel and the larger end sealed with various capping materials including Mineral Trioxide Aggregate (MTA), Biodentine, hydroxyapatite-tricalcium phosphate (HA-TCP), composite, and glass ionomer. The tube was placed in wells of culture plates and incubated for various times in vitro and observed under the microscope for cell morphological changes. pH changes within the tube were monitored over time.
resultsBoth fresh MTA and Biodentine caused adverse response to the cells in the tube. Only a few normally growing cells were observed at the apical end. Composite or glass ionomer appeared better tolerated by cells. HA-TCP mixed in fibrin gel demonstrated the highest compatibility with cells; however, using HA-TCP to separate cells from fresh MTA or Biodentine did not reduce the negative effect of these 2 calcium silicate cements. The pH increased within the tube may explain the observed toxicity.
conclusionsUsing HA-TCP mixed in fibrin gel as a capping material appears highly biocompatible to cells while fresh MTA and Biodentine are not.
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Registered trials
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