Evidence map›Paper›PMID 40347336›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Electrophoretic Zymography of Protease Using Azocasein.

Jônatas Carvalho-Silva, Lígia M G Fernandes, Attilio Converti, Tatiana S Porto

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Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Jônatas Carvalho-SilvaDepartament of Morphology and Animal Physiology, Federal Rural University of Pernambuco, Recife, Pernambuco, Brazil.
Lígia M G FernandesDepartament of Morphology and Animal Physiology, Federal Rural University of Pernambuco, Recife, Pernambuco, Brazil.
Attilio ConvertiDepartment of Civil, Chemical and Environmental Engineering, University of Genova, Genoa, Italy.
Tatiana S PortoDepartament of Morphology and Animal Physiology, Federal Rural University of Pernambuco, Recife, Pernambuco, Brazil. tatiana.porto@ufrpe.br.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Zymography is an electrophoretic technique used to demonstrate enzyme activity during separation in polyacrylamide gels under nonreducing conditions. This kind of analysis can be used in association with electrophoresis (SDS-PAGE) to show which protein is correlated with a given activity. Proteases are the most used enzymes for this technique since the polyacrylamide gel can be impregnated with azocasein, a casein derivative with an added sulfanilamide group (orange color). Furthermore, azocasein can be degraded while the protease is separated through the gel. The degradation is easily revealed by dyeing the azocasein with Coomassie blue, with the nonblue area in the gel being the zone where the separated protease shows activity. Zymography has been widely used for the identification and characterization of new proteolytic enzymes, but it can detect latent and active forms of enzymes in cells, tissues, or biological fluids.

Indexed as

Electrophoresis, Polyacrylamide GelEnzyme AssaysPeptide HydrolasesCaseinsRosaniline DyesCaseinsPeptide HydrolasesRosaniline DyesAzocaseinElectrophoresisPolyacrylamide gelProteolytic enzyme

Identifiers

PMID40347336

What OpenQuestion holds

Textmetadata
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.