Evidence map›Paper›PMID 40347332›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Measuring Matrix Metalloproteinases Activity by Gelatin Zymography.

Kelli C Micocci, Uliana S Stotzer, Milene N O Moritz

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Kelli C MicocciDepartment of Chemistry, Federal University of São Carlos, Sao Paulo, Brazil.
Uliana S StotzerHuman Movement Sciences Research Group, Methodist University of Piracicaba, Sao Paulo, Brazil.
Milene N O MoritzSao Carlos Institute of Chemistry, University of Sao Paulo, Sao Paulo, Brazil.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Gelatin zymography is a widely popular method due to its simplicity, low cost, and quick results, for studying gelatinases in various biological systems. Zymography can detect both the pro and active forms of matrix metalloproteinases MMP-2 (gelatinase A) and MMP-9 (gelatinase B). These MMPs play critical roles in the pathophysiology of many human diseases, particularly in cancer progression. Gelatin zymography is a method based on a suitable protein substrate incorporated into a sodium dodecyl sulfate-polyacrylamide gel. Substrate degradation by protease-containing samples can be visualized through the contrast between the Coomassie blue-stained gel and the white band of substrate degradation. Here, we provide a straightforward, step-by-step methodology for detecting MMP-2 and MMP-9 gelatinases in tumor cells. It is essential to highlight that accurately interpreting the data requires a thorough understanding of the technique's principles.

Indexed as

Electrophoresis, Polyacrylamide GelEnzyme AssaysGelatinMatrix Metalloproteinase 2Matrix Metalloproteinase 9Matrix MetalloproteinasesCell Line, TumorHumansGelatinMatrix Metalloproteinase 2Matrix Metalloproteinase 9Matrix MetalloproteinasesCell cultureGelatinMatrix metalloproteinasesMMP-2MMP-9Zymography

Identifiers

PMID40347332

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.