Evidence map›Paper›PMID 40347329›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Gelatin Zymography: A Simple and Effective Technique to Detect Gelatinases.

Meilang Xue, Haiyan Lin, Christer J Jackson, Lyn March

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Meilang XueSutton Arthritis Research Laboratory, Sydney Musculoskeletal Health, Kolling Institute, Faculty of Medicine and Health, The University of Sydney, Sydney, NSW, Australia. meilang.xue@sydney.edu.au.
Haiyan LinSutton Arthritis Research Laboratory, Sydney Musculoskeletal Health, Kolling Institute, Faculty of Medicine and Health, The University of Sydney, Sydney, NSW, Australia.
Christer J JacksonSutton Arthritis Research Laboratory, Sydney Musculoskeletal Health, Kolling Institute, Faculty of Medicine and Health, The University of Sydney, Sydney, NSW, Australia.
Lyn MarchThe Australian Arthritis and Autoimmune Biobank Collaborative (A3BC), Sydney Musculoskeletal Health, Kolling Institute, Faculty of Medicine and Health, The University of Sydney, Sydney, NSW, Australia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Matrix metalloproteinases (MMPs) are zinc-dependent enzymes capable of breaking down all extracellular matrix (ECM) components. The gelatinases are a subgroup of MMPs which have two members: gelatinase A (MMP-2) and gelatinase B (MMP-9). They are highly efficient in breaking down denatured collagen I or gelatin. This ability of gelatinases has led to the development of a technique called gelatin zymography. This technique provides a simple, sensitive, and robust way to detect gelatinolytic activity in biological samples. This chapter provides a detailed protocol on how to implement the gelatin zymography and analyze gelatinases in biological samples using this technique.

Indexed as

Electrophoresis, Polyacrylamide GelEnzyme AssaysGelatinGelatinasesAnimalsHumansMatrix Metalloproteinase 2GelatinGelatinasesMatrix Metalloproteinase 2ElectrophoresisGelatinGelatinaseGelatin zymographyMatrix metalloproteinease

Identifiers

PMID40347329

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.