Evidence map›Paper›PMID 40347327›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Gelatin Zymography: Principle, Method and Application in Cancer Research.

Luomeng Qian, Sihe Zhang

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Luomeng QianDepartment of Cell Biology, School of Medicine, Nankai University, Tianjin, China.
Sihe ZhangDepartment of Cell Biology, School of Medicine, Nankai University, Tianjin, China. sihezhang@nankai.edu.cn.ORCID https://orcid.org/0000-0002-8923-1993

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Gelatin zymography is a simple semiquantitative technique for assaying gelatinases in various biological samples. It is particularly feasible to measure two gelatin-degrading matrix metalloproteinases, MMP-2 (gelatinase A) and MMP-9 (gelatinase B). The use of SDS-polyacrylamide gels impregnated with gelatin provides a reliable way to detect and quantify the type, amount, and activation level of gelatinases in biological samples (cell lysates, tissue extracts, and biological fluids). This method can be used to investigate the expression profile and activation level of gelatinase in cancer cells or tissues and to determine how these changes are related to cancer progression. However, it is crucial to understand the principle and limitation of gelatin zymography before interpreting the experimental data. Gelatin zymography is a powerful method for assaying gelatinases in cancer research if some precautions are taken.

Indexed as

Electrophoresis, Polyacrylamide GelEnzyme AssaysGelatinGelatinasesNeoplasmsAnimalsHumansMatrix Metalloproteinase 2Matrix Metalloproteinase 9GelatinGelatinasesMatrix Metalloproteinase 2Matrix Metalloproteinase 9Activation profileGelatinaseGelatin zymographyGel electrophoresisMatrix metalloproteinase

Identifiers

PMID40347327

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.