Evidence map›Paper›PMID 40345598›Full record

ArticleJournal of virological methods2025

Effect of temperature and time on RNA detection by RT-qPCR in rodent tissue and blood samples stored in MagMAX™ Lysis/Binding Solution Concentrate: Considerations for RNA detection in specimens stored under suboptimal conditions.

Katherine A Davies, Stephen R Welch, Teresa E Sorvillo, JoAnn D Coleman-McCray, Christina F Spiropoulou, Jessica R Spengler

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of virological methods, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Katherine A DaviesViral Special Pathogens Branch, Division of High-Consequence Pathogens and Pathology, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA; Zoonotic and Emerging Disease Research Unit, National Bio and Agro-Defense Facility, Agricultural Research Service, United States Department of Agriculture, Manhattan, KS 66506, USA.
Stephen R WelchViral Special Pathogens Branch, Division of High-Consequence Pathogens and Pathology, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.
Teresa E SorvilloViral Special Pathogens Branch, Division of High-Consequence Pathogens and Pathology, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA; Infectious Disease Department, CDC Foundation, Atlanta, GA, 30308, USA.
JoAnn D Coleman-McCrayViral Special Pathogens Branch, Division of High-Consequence Pathogens and Pathology, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.
Christina F SpiropoulouViral Special Pathogens Branch, Division of High-Consequence Pathogens and Pathology, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.
Jessica R SpenglerViral Special Pathogens Branch, Division of High-Consequence Pathogens and Pathology, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA. Electronic address: wsk7@cdc.gov.

Funding

CDC HHSIntramural CDC HHS CC999999USDAUS Department of Energy
6 · The paper itself

Abstract

For detection of viral RNA in blood or tissue, samples are often collected into lysis buffers prior to downstream molecular analysis. Immediate sample processing and cold storage are not always possible during large-scale or field studies, or in facilities lacking a stable electrical supply. Additionally, samples may need to be transported significant distances before processing. Here, using peptidylprolyl isomerase A (Ppia), a stably expressed gene in rodent tissues, we investigate the long-term stability and detection of RNA in guinea pig tissues stored for up to 52 weeks and in hamster blood stored for up to 12 weeks in MagMAX Lysis/Binding Solution Concentrate at -80°C, 4°C, 21°C, and 32°C.

Indexed as

Real-Time Polymerase Chain ReactionRNA, ViralSpecimen HandlingAnimalsCricetinaeGuinea PigsTemperatureTime FactorsRNA, ViralLysis bufferRNA stabilityStorageTemperature

Identifiers

PMID40345598
PMCPMC12232495

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.