ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2025
In-Depth Cell-Type-Specific Proteome Landscape of the Brain from Human Amyloid-β Overexpression Mouse Model.
Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
2 citing papers in PubMed.
- Cell type-resolved proteomics reveals intra- and intercellular signaling in Alzheimer's disease.bioRxiv : the preprint server for biology · 2026Article
- In-Depth Cell-Type-Specific Proteome Landscape of the Brain from Human Amyloid-β Overexpression Mouse Model.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
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Authors and funding
7 authors.
Funding
Abstract
Amyloid-β (Aβ) plays a crucial role in Alzheimer's disease pathogenesis. Understanding how Aβ overexpression alters the proteome of individual brain cell types is essential but challenging due to the nature of brain tissue, which contains intermingled various cell types. The current methods for cell-type-specific proteomics either require genetic modifications or complex cell isolation, limiting their use. This study introduces a novel method, in situ cell-type-specific proteome analysis using antibody-mediated biotinylation (iCAB), which applies immunohistochemistry with biotin-tyramide to target cell-specific proteins directly in tissue. Applied to 5xFAD mice, iCAB enables us to identify ≈8000 cell-type-specific proteomes with significantly more differentially expressed proteins than traditional bulk proteome methods, pinpointing unique pathways such as mRNA processing, calcium regulation, and phagocytosis for neurons, astrocytes, and microglia, respectively. This study reports in-depth the cell-type-specific brain proteome landscape of the human Aβ overexpression mouse model for the first time using an innovative tool that is powerful, straightforward, and applicable to both animal models and human tissues, without the need for prior genetic alterations.
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