Evidence map›Paper›PMID 40344631›Full record

ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2025

A Phase-Separated SR Protein Reprograms Host Pre-mRNA Splicing to Enhance Disease Susceptibility.

Dong Yan, Jie Huang, Fengqi Tian, Haidong Shu, Han Chen, Qian Peng, Hongwei Wu, Jianlong Zhao, Anireddy S N Reddy, Gang Li and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Article
  2. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Dong YanState Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, Nanjing Agricultural University, Nanjing, 210095, China.
Jie HuangThe Plant Chemetics Laboratory, Department of Biology, University of Oxford, Oxford, OX1 3RB, UK.ORCID https://orcid.org/0000-0002-6657-8740
Fengqi TianState Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, Nanjing Agricultural University, Nanjing, 210095, China.
Haidong ShuState Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, Nanjing Agricultural University, Nanjing, 210095, China.
Han ChenState Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, Nanjing Agricultural University, Nanjing, 210095, China.
Qian PengState Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, Nanjing Agricultural University, Nanjing, 210095, China.
Hongwei WuState Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, Nanjing Agricultural University, Nanjing, 210095, China.
Jianlong ZhaoState Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Anireddy S N ReddyDepartment of Biology and Program in Cell and Molecular Biology, Colorado State University, Fort Collins, CO, 80523, USA.
Gang LiState Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, Nanjing Agricultural University, Nanjing, 210095, China.
Yuanchao WangState Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, Nanjing Agricultural University, Nanjing, 210095, China.
Suomeng DongState Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, Nanjing Agricultural University, Nanjing, 210095, China.

Funding

Agriculture Research System of China CARS-09-P20Agriculture Research System of China CARS-potatoChina Postdoctoral Science Foundation 2020T130306European Research Council (ERC) Advanced Grants ExtraImmune,101019324Guangdong Major Project of Basic and Applied Basic Research 2021B0301030004National Natural Science Foundation of China 32130088National Natural Science Foundation of China 32488302
6 · The paper itself

Abstract

Alternative splicing (AS) plays a vital role in the plant-microbe interaction. Modulating host precursor-mRNA AS is a key strategy employed by multiple pathogens to subvert plant immunity. However, the underlying mechanism by which the host splicing factor regulates plant immunity remains poorly understood. Here, a plant-conserved serine/arginine-rich (SR) RNA splicing factor, SR30, which negatively regulates tomato immunity against the infamous Phytophthora infestans (P. infestans) is identified. SR30 governs tomato mRNA AS at a genome-wide level and suppresses defense-related genes AS. During P. infestans infection, SR30 is induced to form nuclear condensates via liquid-liquid phase separation driven by intrinsically disordered regions. Importantly, the phase separation property is required for the function of SR30 in disease susceptibility and the regulation of genes AS. Knockout of SR30 via CRISPR/Cas9 improves tomato disease resistance to P. infestans, P. capsici, and P. parasitica by promoting defense genes AS. These findings uncover a novel mechanism in a phase-separated protein that regulates plant immunity by altering the AS of defense-related genes and provides a new paradigm for engineering protein condensate in crop-resistant breeding.

Indexed as

Plant DiseasesPlant ImmunityPlant ProteinsRNA PrecursorsSolanum lycopersicumAlternative SplicingDisease ResistanceDisease SusceptibilityGene Expression Regulation, PlantPhytophthora infestansRNA SplicingPlant ProteinsRNA Precursorsalternative splicinglate blight diseasephase separationplant immunitySR proteins

Identifiers

PMID40344631
PMCPMC12279203

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.