Evidence map›Paper›PMID 40344613›Full record

ArticleThe New phytologist2025

Hypoxia-activated fluorescent probes as markers of oxygen levels in plant cells and tissues.

Monica Perri, M Shahneawz Khan, Antoine L D Wallabregue, Viktoriia Voloboeva, Amber M Ridgway, Edward N Smith, Hannah Bolland, Ester M Hammond, Stuart J Conway, Daan A Weits and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The New phytologist, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Monica Perri *Department of Biology, University of Oxford, South Parks Road, Oxford, OX1 3RB, UK.ORCID https://orcid.org/0000-0001-6955-4691
M Shahneawz Khan *Department of Biology, University of Oxford, South Parks Road, Oxford, OX1 3RB, UK.ORCID https://orcid.org/0000-0002-0141-1827
Antoine L D Wallabregue *Department of Chemistry, Chemistry Research Laboratory, University of Oxford, Mansfield Road, Oxford, OX1 3TA, UK.ORCID https://orcid.org/0000-0002-3181-8636
Viktoriia VoloboevaExperimental and Computational Plant Development, Institute of Environment Biology, Utrecht University, Padualaan 8, Utrecht, 3584 CH, the Netherlands.
Amber M RidgwayDepartment of Chemistry, Chemistry Research Laboratory, University of Oxford, Mansfield Road, Oxford, OX1 3TA, UK.
Edward N SmithDepartment of Biology, University of Oxford, South Parks Road, Oxford, OX1 3RB, UK.
Hannah BollandDepartment of Oncology, University of Oxford, Old Road Campus Research Building, Oxford, OX3 7DQ, UK.
Ester M HammondDepartment of Oncology, University of Oxford, Old Road Campus Research Building, Oxford, OX3 7DQ, UK.
Stuart J ConwayDepartment of Chemistry, Chemistry Research Laboratory, University of Oxford, Mansfield Road, Oxford, OX1 3TA, UK.
Daan A WeitsExperimental and Computational Plant Development, Institute of Environment Biology, Utrecht University, Padualaan 8, Utrecht, 3584 CH, the Netherlands.ORCID https://orcid.org/0000-0003-4423-5568
Emily FlashmanDepartment of Biology, University of Oxford, South Parks Road, Oxford, OX1 3RB, UK.ORCID https://orcid.org/0000-0002-4169-4278

Funding

Biotechnology and Biological Sciences Research Council BB/T008784/1Engineering and Physical Sciences Research Council EP/S019901/1European Research Council starting grant LOKI 101077812H2020 European Research Council 864888
6 · The paper itself

Abstract

Low oxygen signalling in plants is important in development and stress responses. Measurement of oxygen levels in plant cells and tissues is hampered by a lack of chemical tools with which to reliably detect and quantify endogenous oxygen availability. We have exploited hypoxia-activated fluorescent probes to visualise low oxygen (hypoxia) in plant cells and tissues. We applied 4-nitrobenzyl (4NB-) resorufin and methyl-indolequinone (MeIQ-) resorufin to Arabidopsis thaliana whole cells and seedlings exposed to hypoxia (1% O

Indexed as

ArabidopsisFluorescent DyesOxygenPlant CellsBiomarkersCell HypoxiaIndolequinonesMicroscopy, ConfocalOxazinesPlant LeavesPlant RootsSeedlingsBiomarkersFluorescent DyesIndolequinonesOxazinesOxygenresorufinbioreductionfluorogenic probeshypoxiaoxygen‐sensingResorufin

Identifiers

PMID40344613
PMCPMC12371156

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.