Evidence map›Paper›PMID 40343949›Full record

ArticlePloS one2025

Performance comparison of streptavidin magnetic beads for epcam expressing cancer cell lines for circulating tumor cell (CTC) enrichment in a flow-through immunomagnetic system.

Peng Liu, Sitian He, Pieter Hart, Alloïse van Kleef, Stan Boxum, Anouk Mentink, Yongjun Wu, Leon W M M Terstappen, Pascal Jonkheijm, Michiel Stevens

Abstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Peng LiuDepartment of Medical Cell Biophysics, Techmed Center, Faculty of Science and Technology, University of Twente, Enschede, The Netherlands.
Sitian HeDepartment of Medical Cell Biophysics, Techmed Center, Faculty of Science and Technology, University of Twente, Enschede, The Netherlands.
Pieter HartDepartment of Medical Cell Biophysics, Techmed Center, Faculty of Science and Technology, University of Twente, Enschede, The Netherlands.
Alloïse van KleefDepartment of Medical Cell Biophysics, Techmed Center, Faculty of Science and Technology, University of Twente, Enschede, The Netherlands.
Stan BoxumDepartment of Medical Cell Biophysics, Techmed Center, Faculty of Science and Technology, University of Twente, Enschede, The Netherlands.
Anouk MentinkDepartment of Medical Cell Biophysics, Techmed Center, Faculty of Science and Technology, University of Twente, Enschede, The Netherlands.
Yongjun WuCollege of Public Health, Zhengzhou University, Zhengzhou, China.
Leon W M M TerstappenDepartment of Medical Cell Biophysics, Techmed Center, Faculty of Science and Technology, University of Twente, Enschede, The Netherlands.
Pascal JonkheijmDepartment of Molecules and Materials, Laboratory of Biointerface Chemistry and the TechMed Centre, University of Twente, Enschede, The Netherlands.
Michiel StevensDepartment of Medical Cell Biophysics, Techmed Center, Faculty of Science and Technology, University of Twente, Enschede, The Netherlands.ORCID https://orcid.org/0000-0001-8643-7235

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Circulating tumor cells (CTCs) are important biomarkers for cancer diagnosis and treatment monitoring. However, their scarcity limits their utility as current enrichment techniques are hampered by low volume throughput and/or the inability to capture CTCs with low target antigen densities. Our group previously reported a device capable of processing samples in a flow manner using an optimized Halbach array to enhance the capture of low EpCAM-expressing cells (Flow-through Immunomagnetic CTC Enrichment system). In this study, we tested the capture efficiency of eight commercially available streptavidin magnetic beads using this device to identify the most suitable bead. Results indicate that using this system, the best-performing magnetic beads are in the ~ 100 to ~ 150 nm size range. Considering the combination of binding efficiency and final sample purity, we found that among the beads tested in combination with biotinylated anti-EpCAM, MojoSort Streptavidin Nanobeads performed the best, with high capture efficiencies for both the high EpCAM expressing LNCaP and low EpCAM expressing PC3-9 cell lines. For CTC enrichment from the blood of cancer patients, reducing the number of WBCs co-enriched with these beads will be essential, especially when processing large-volume samples acquired, for instance, through diagnostic leukapheresis to overcome the limitations caused by the scarcity of CTCs.

Indexed as

Antigens, NeoplasmCell Adhesion MoleculesImmunomagnetic SeparationNeoplastic Cells, CirculatingStreptavidinCell Line, TumorEpithelial Cell Adhesion MoleculeHumansAntigens, NeoplasmCell Adhesion MoleculesEPCAM protein, humanEpithelial Cell Adhesion MoleculeStreptavidin

Identifiers

PMID40343949
PMCPMC12063838

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.