Transcriptomic insights into methanol utilization in Pichia pastoris lacking AOX genes under co-feeding conditions.

Xueyun Zheng, Zhifang Ye, Jiao Gao, Yuechuo Hao, Cheng Li, Hongsen Xie, Ying Lin, Shuli Liang

Abstract read

In one paragraph

Article in Archives of microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed

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1 · What the graph read from it

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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0 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

8 authors.

Xueyun Zheng *Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei University of Technology, Wuhan, 430068, China.

Zhifang Ye *Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei University of Technology, Wuhan, 430068, China.

Jiao GaoGuangdong Key Laboratory of Fermentation and Enzyme Engineering, South China University of Technology, Guangzhou, 510006, China.

Yuechuo HaoGuangdong Key Laboratory of Fermentation and Enzyme Engineering, South China University of Technology, Guangzhou, 510006, China.

Cheng LiDepartment of Biology, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.

Hongsen XieKey Laboratory of Fermentation Engineering (Ministry of Education), Hubei University of Technology, Wuhan, 430068, China.

Ying LinGuangdong Key Laboratory of Fermentation and Enzyme Engineering, South China University of Technology, Guangzhou, 510006, China. feylin@scut.edu.cn.

Shuli LiangGuangdong Key Laboratory of Fermentation and Enzyme Engineering, South China University of Technology, Guangzhou, 510006, China. shuli@scut.edu.cn.

Funding

National Natural Science Foundation of China 32272276Natural Science Foundation of Hubei Province 2024AFD151the National Key Research and Development Program 2021YFC2100400

6 · The paper itself

Abstract

The methylotrophic yeast Pichia pastoris (P. pastoris) exhibits remarkable capability for methanol-driven protein biosynthesis, positioning it as an attractive platform for carbon-neutral biomanufacturing utilizing methanol as a renewable feedstock. However, challenges arising from methanol metabolism, particularly the accumulation of toxic formaldehyde intermediates, significantly hinder efficient methanol biotransformation. To address this limitation, we implemented a metabolic engineering strategy involving dual knockout of alcohol oxidase genes (aox1 and aox2) combined with glycerol co-substrate supplementation. Using enhanced green fluorescent protein (EGFP) as a model heterologous product, we demonstrated that the ΔAOX1/2 strain achieved superior protein productivity in glycerol-methanol co-feeding cultures. Under optimized conditions (0.5% methanol + 0.4% glycerol), the engineered strain attained a biomass density of 38.5 (OD

Indexed as

Alcohol OxidoreductasesFungal ProteinsMethanolPichiaSaccharomycetalesTranscriptomeBiomassGene Expression ProfilingGlycerolGreen Fluorescent ProteinsMetabolic Engineeringalcohol oxidaseAlcohol OxidoreductasesFungal ProteinsGlycerolGreen Fluorescent ProteinsMethanolMethanol utilizationPichia pastorisProtein productionTranscriptome analysis

Identifiers

PMID40343512

DOI10.1007/s10295-003-0035-3

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.