Evidence map›Paper›PMID 40343338›Full record

ArticleResearch square2025

Cryo-EM reveals remodeling of a tandem riboswitch at 2.9 Å resolution.

Nathan Jespersen, Jigneshkumar Dahyabhai Prajapati, Ankush Singhal, Karissa Y Sanbonmatsu

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in Research square, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Nathan JespersenTheoretical Biology and Biophysics, Los Alamos National Laboratory, Los Alamos, NM 87545, United States.
Jigneshkumar Dahyabhai PrajapatiTheoretical Biology and Biophysics, Los Alamos National Laboratory, Los Alamos, NM 87545, United States.
Ankush SinghalTheoretical Biology and Biophysics, Los Alamos National Laboratory, Los Alamos, NM 87545, United States.
Karissa Y SanbonmatsuTheoretical Biology and Biophysics, Los Alamos National Laboratory, Los Alamos, NM 87545, United States.ORCID https://orcid.org/0000-0002-7965-7392

Funding

NCCAT: National Center for CryoEM Access and Training- Supplement for Windows 10 and FFIU24GM129539 · NIGMS · NEW YORK STRUCTURAL BIOLOGY CENTER · PI DE MARCO, ALEX, KIEFT, JEFFREY S · 2018 to 2023
$53.9M
NCCAT: National Center for CryoEM Access and TrainingR24GM154192 · NIGMS · NEW YORK STRUCTURAL BIOLOGY CENTER · PI EDWARD T ENG, Jeffrey S Kieft · 2024 to 2026
$21.0M
Structure-based Simulation of Riboswitches: Electrostatic EffectsR01GM110310 · NIGMS · TRIAD NATIONAL SECURITY, LLC · PI SANBONMATSU, KARISSA Y · 2015 to 2023
$2.6M
NIGMS NIH HHS R01 GM110310NIGMS NIH HHS R24 GM154192NIGMS NIH HHS U24 GM129539
6 · The paper itself

Abstract

Riboswitches are non-coding RNA sequences that control cellular processes through ligand binding. Conformational heterogeneity is fundamental to riboswitch functionality, yet this same attribute makes structural characterization of these mRNA elements challenging. Here, we use a combination of molecular dynamics and cryo-electron microscopy to expound the flexible nature of the glycine riboswitch tandem aptamers and characterize diMerent structural populations. We find that Mg

Identifiers

PMID40343338
PMCPMC12060979

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.