ArticleEquine veterinary journal2026
Synovial fluid alpha-2-macroglobulin, gelsolin and lubricin distinguish between osteoarthritic and healthy equine joints.
Article in Equine veterinary journal, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed.
- Evidence for alpha-2-macroglobulin as an orthobiologic osteoarthritis therapy: a narrative review.American journal of veterinary research · 2026Review
- Alpha2EQ downregulates proinflammatory cytokine and chemokine gene expression in cultured synovial fibroblasts.American journal of veterinary research · 2026Article
- Synovial fluid alpha-2-macroglobulin, gelsolin and lubricin distinguish between osteoarthritic and healthy equine joints.Equine veterinary journal · 2026Article
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Authors and funding
5 authors.
Funding
Abstract
backgroundSynovial fluid (SF) is an ideal sentinel fluid for osteoarthritis (OA) diagnosis and prognostication due to its critical homeostatic role, proximity to articular tissues and immune cell composition. Untargeted proteomics enable identification of soluble markers for diagnostic and therapeutic applications while minimising bias.
objectivesTo use liquid chromatography-tandem mass spectrometry (LC-MS/MS) to define the SF proteome in horses with and without carpal OA. The goal was to identify differentially regulated proteins in mild-moderate carpal joint disease compared with healthy joints. STUDY
designCross-sectional study.
methodsSynovial fluid was obtained from horses undergoing arthroscopic treatment for carpal fragmentation and from horses with healthy carpal joints. LC-MS/MS proteomics was performed on a subset of joints (n = 8 OA, n = 8 healthy). Total protein (TP), gelsolin, lubricin and prostaglandin E
resultsOf 119 proteins identified, 14 were increased and 10 decreased in OA. Two of the most upregulated proteins included pregnancy zone protein and alpha-2-macroglobulin. In OA, gelsolin/TP was decreased (OA median: 0.48 μg/mL/mg [95% confidence interval 0.27-0.73]; healthy: 0.89 μg/mL/mg [0.63-1.17]) and lubricin/TP increased (OA: 27.98 μg/mL/mg, [21.71-45.79]; healthy: 6.77 μg/mL/mg [4.36-9.94]) when measured by immunoassay. Multivariate modelling including gelsolin/TP, lubricin/TP and PGE MAIN LIMITATIONS: Synovial membrane was available for a limited number of joints and most healthy samples were collected following euthanasia.
conclusionsThe upregulation of pregnancy zone protein and alpha-2-macroglobulin in OA joints motivates investigation into their function in OA and development of reagents for quantification. Several proteins with differential abundance in OA SF, including gelsolin and lubricin, were measured and may have diagnostic utility.
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