Evidence map›Paper›PMID 40341961›Full record

ArticleGenomics, proteomics & bioinformatics2025

rMATS-cloud: Large-scale Alternative Splicing Analysis in the Cloud.

Jenea I Adams, Eric Kutschera, Qiang Hu, Chun-Jie Liu, Qian Liu, Kathryn Kadash-Edmondson, Song Liu, Yi Xing

Abstract read
In one paragraph

Article in Genomics, proteomics & bioinformatics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Jenea I AdamsGenomics and Computational Biology Graduate Group, University of Pennsylvania, Philadelphia, PA 19104, USA.ORCID 0000-0002-2590-3594
Eric KutscheraCenter for Computational and Genomic Medicine, The Children's Hospital of Philadelphia, Philadelphia, PA 19104, USA.ORCID 0000-0002-2160-1820
Qiang HuDepartment of Biostatistics and Bioinformatics, Roswell Park Comprehensive Cancer Center, Buffalo, NY 14203, USA.ORCID 0000-0002-4090-5539
Chun-Jie LiuCenter for Computational and Genomic Medicine, The Children's Hospital of Philadelphia, Philadelphia, PA 19104, USA.ORCID 0000-0002-3008-3375
Qian LiuDepartment of Biostatistics and Bioinformatics, Roswell Park Comprehensive Cancer Center, Buffalo, NY 14203, USA.ORCID 0000-0003-1456-5099
Kathryn Kadash-EdmondsonCenter for Computational and Genomic Medicine, The Children's Hospital of Philadelphia, Philadelphia, PA 19104, USA.ORCID 0000-0002-1437-0147
Song LiuDepartment of Biostatistics and Bioinformatics, Roswell Park Comprehensive Cancer Center, Buffalo, NY 14203, USA.ORCID 0000-0001-6351-2941
Yi XingCenter for Computational and Genomic Medicine, The Children's Hospital of Philadelphia, Philadelphia, PA 19104, USA.ORCID 0000-0001-9257-7613

Funding

UNIVERSITY OF PENNSYLVANIA CAN CTR SUPPORT GRANTP30CA016520 · NCI · UNIVERSITY OF PENNSYLVANIA · PI SHELLEY L BERGER · 1985 to 2026
$222.3M
Immuno-Oncology Translation Network: Data Management and Resource-Sharing Center at RPCIU24CA232979 · NCI · ROSWELL PARK CANCER INSTITUTE CORP · PI HUTSON, ALAN DAVID, LIU, SONG · 2018 to 2024
$8.9M
YAP1 and RB1 cooperate to regulate lung cancer lineage plasticity and therapeutic resistanceU24CA274159 · NCI · ROSWELL PARK CANCER INSTITUTE CORP · PI DAVID W. GOODRICH, Alan David Hutson · 2022 to 2026
$8.5M
Targeting alternative splicing for TCR discovery in small cell carcinomasU01CA233074 · NCI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI CROOKS, GAY M, WITTE, OWEN N. · 2018 to 2022
$4.1M
VARIATION AND REGULATION OF ALTERNATIVE SPLICING IN HUMAN TRANSCRIPTOMESR01GM088342 · NIGMS · UNIVERSITY OF IOWA · PI XING, YI · 2010 to 2020
$2.9M
Targeting alternative isoform variation for TCR discovery in platinum-resistant ovarian cancerR01CA287673 · NCI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Sanaz Memarzadeh, Yi Xing · 2024 to 2026
$2.1M
ProteoSeq - An Integrative Computational Framework for ProteotranscriptomicsR01GM117624 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI XING, YI · 2016 to 2018
$1.1M
NCI NIH HHS P30 CA016520NCI NIH HHS R01 CA287673NCI NIH HHS U01 CA233074NCI NIH HHS U24 CA232979NCI NIH HHS U24 CA274159NIGMS NIH HHS R01 GM088342NIGMS NIH HHS R01 GM117624NIH HHS P30CA016520-47S3NIH HHS R01CA287673NIH HHS R01GM088342NIH HHS R01GM117624NIH HHS U01CA233074NIH HHS U24CA232979NIH HHS U24CA274159
6 · The paper itself

Abstract

Although gene expression analysis pipelines are often a standard part of bioinformatics analysis, with many publicly available cloud workflows, cloud-based alternative splicing analysis tools remain limited. Our lab released rMATS in 2014 and has continuously maintained it, providing a fast and versatile solution for quantifying alternative splicing from RNA sequencing (RNA-seq) data. Here, we present rMATS-cloud, a portable version of the rMATS workflow that can be run in virtually any cloud environment suited for biomedical research. We compared the time and cost of running rMATS-cloud with two RNA-seq datasets on three different platforms (Cavatica, Terra, and Seqera). Our findings demonstrate that rMATS-cloud handles RNA-seq datasets with thousands of samples, and therefore is ideally suited for the storage capacities of many cloud data repositories. rMATS-cloud is available at https://dockstore.org/workflows/github.com/Xinglab/rmats-turbo/rmats-turbo-cwl, https://dockstore.org/workflows/github.com/Xinglab/rmats-turbo/rmats-turbo-wdl, and https://dockstore.org/workflows/github.com/Xinglab/rmats-turbo/rmats-turbo-nextflow.

Indexed as

Alternative SplicingCloud ComputingComputational BiologySoftwareHumansRNA-SeqSequence Analysis, RNAAlternative splicingBioinformaticsCloud computingRNA-seqTranscriptomics

Identifiers

PMID40341961
PMCPMC12248417

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.