Double alkylation with maleimide-PEG-biotin: An enrichment method for cysteine redox states.

Jung Mi Lim, Rodney L Levine

Abstract read

In one paragraph

Article in Analytical biochemistry, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

2 authors.

Jung Mi LimLaboratory of Biochemistry, National Heart, Lung, and Blood Institute, Building 50, Room 2347, 50 South Drive, MSC 8012, Bethesda, 20892-8012, Maryland, USA. Electronic address: jungmi.lim@nih.gov.

Rodney L LevineLaboratory of Biochemistry, National Heart, Lung, and Blood Institute, Building 50, Room 2351, 50 South Drive, MSC 8012, Bethesda, 20892-8012, Maryland, USA. Electronic address: rlevine@nih.gov.

Funding

Oxidative Modification Of ProteinsZIAHL000225 · NHLBI · NATIONAL HEART, LUNG, AND BLOOD INSTITUTE · PI LEVINE, RODNEY L. · 2009 to 2025

$35.2M

Intramural NIH HHS ZIA HL000225

6 · The paper itself

Abstract

Cysteine alkylation is widely used in mass-spectrometric based proteomic studies. The oxidation state of each cysteine can be determined by labeling free thiols with one alkylating agent and disulfides with a second alkylating agent that differs in mass from the first. We have developed an improved method utilizing biotin-conjugated maleimides to specifically label cysteine residues in the thiol state and in disulfide linkage. The biotin tag effectuates very efficient enrichment of cysteine containing peptides, greatly increasing sensitivity for those peptides. We also achieve very high recovery of the biotinylated peptides from an avidin column by elution with hexafluoro-2-propanol (HFIP). The method offers improved mapping of the cysteine proteome and its oxidation state.

Indexed as

BiotinCysteineMaleimidesPolyethylene GlycolsAlkylationOxidation-ReductionBiotinCysteinemaleimideMaleimidesPolyethylene Glycols

Identifiers

PMID40339779

DOI10.1016/j.ab.2025.115892

PMCPMC12145231

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.