Evidence map›Paper›PMID 40338290›Full record

ArticleJournal of assisted reproduction and genetics2025

Agouti-signaling protein (ASIP) improves bovine in vitro matured oocyte developmental competence and modulates lipid content.

Heather L Chaney, Jaelyn Z Current, Mingxiang Zhang, Jianbo Yao

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Article in Journal of assisted reproduction and genetics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

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3 · Its place in the literature

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1 citing paper in PubMed.

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4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Heather L ChaneyDivision of Animal and Nutritional Sciences, Davis College of Agriculture, Natural Resources and Design, West Virginia University, Morgantown, WV, USA.
Jaelyn Z CurrentDivision of Animal and Nutritional Sciences, Davis College of Agriculture, Natural Resources and Design, West Virginia University, Morgantown, WV, USA.
Mingxiang ZhangDivision of Animal and Nutritional Sciences, Davis College of Agriculture, Natural Resources and Design, West Virginia University, Morgantown, WV, USA.
Jianbo YaoDivision of Animal and Nutritional Sciences, Davis College of Agriculture, Natural Resources and Design, West Virginia University, Morgantown, WV, USA. jianbo.yao@mail.wvu.edu.ORCID http://orcid.org/0000-0001-8419-7290

Funding

National Institute of Food and Agriculture GNE21-252-35383
6 · The paper itself

Abstract

purposeBovine embryos produced in vitro are developmentally inferior compared to in vivo derived embryos due to the lack of optimization of the oocyte and embryo culture conditions in vitro. Agouti-signaling protein (ASIP), a secreted protein produced by the bovine oocyte, has been recently shown to aid in acquiring oocyte developmental competence. Therefore, in the present study, we aimed to reveal the effects of supplementation of ASIP during in vitro oocyte maturation and embryo culture on subsequent embryonic development.

methodsCumulus-oocyte complexes or presumptive zygotes were placed in culture medium containing either 0, 1, 10, or 100 ng/mL of recombinant ASIP (rASIP). Effects on development, gene expression, lipid content, and blastocyst cell allocation were examined.

resultsSupplementation of rASIP during oocyte maturation was found to significantly increase the blastocyst development rate (P < 0.05) and produced blastocysts with an increased inner cell mass to trophectoderm cell ratio. Addition of rASIP during oocyte maturation increased oocyte (P < 0.05) but not embryo (P > 0.05) lipid levels. The expression of genes involved in lipid metabolism, including FASN, PPAR

conclusionsThe data presented in this study further support the role of ASIP in oocyte competence and suggest that the supplementation of rASIP during oocyte maturation may lead to the production of blastocyst of increased quality.

Indexed as

Embryonic DevelopmentIn Vitro Oocyte Maturation TechniquesLipid MetabolismOocytesAnimalsBlastocystCattleCumulus CellsEmbryo Culture TechniquesFemaleFertilization in VitroGene Expression Regulation, DevelopmentalAgouti-signaling proteinDevelopmental competenceEarly embryonic developmentOocyte maturation

Identifiers

PMID40338290
PMCPMC12229579

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