Lowering Ionic Strength Improves the Sensitivity of Microtubule Gliding Assay Based Molecular Detection.

Eugene Christo V R, Esther Charlotte Sophia Kloth, Filippo Nisini, Cordula Reuther, Stefan Diez

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In one paragraph

Article in Nano letters, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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5 · Who and what money

Authors and funding

5 authors.

Eugene Christo V RB CUBE - Center for Molecular Bioengineering, TUD Dresden University of Technology, 01307 Dresden, Germany.ORCID 0009-0000-4306-0319

Esther Charlotte Sophia KlothB CUBE - Center for Molecular Bioengineering, TUD Dresden University of Technology, 01307 Dresden, Germany.

Filippo NisiniB CUBE - Center for Molecular Bioengineering, TUD Dresden University of Technology, 01307 Dresden, Germany.

Cordula ReutherB CUBE - Center for Molecular Bioengineering, TUD Dresden University of Technology, 01307 Dresden, Germany.

Stefan DiezB CUBE - Center for Molecular Bioengineering, TUD Dresden University of Technology, 01307 Dresden, Germany.ORCID 0000-0002-0750-8515

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Microtubule gliding assays provide a unique mechanism for molecular detection in which binding of analytes to the microtubule lattice reduces the microtubule gliding speed. The reduction in the gliding speed correlates with the density of the bound analytes, enabling its quantification. Although promising, this technique is still in the proof-of-concept stage. Improving the sensitivity and limit of detection of the assay could make the technique comparable to that of advanced molecular detection methods. This study demonstrates that reducing the ionic strength of the buffer increases the sensitivity of the assay by enhancing the interactions between kinesin and microtubules. When using a low ionic strength buffer (BRB10) compared with a standard buffer (BRB80), we observed a more pronounced reduction in microtubule gliding speed in the presence of analytes, improving the detection limit. Therefore, this approach offers a simple and scalable way to improve the sensitivity of motor-based detection assays.

Indexed as

KinesinsMicrotubulesAnimalsLimit of DetectionOsmolar ConcentrationKinesinsAssay sensitivityGliding motility assayKinesinMicrotubuleMolecular detectionPoint-of-care diagnostics

Identifiers

PMID40336350

DOI10.1021/acs.nanolett.5c01188

PMCPMC12100704

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.