Evidence map›Paper›PMID 40332426›Full record

ArticleInternational journal of molecular sciences2025

MicroRNA Sequencing of Serum Exosomes Reveals miR205-5p as an Anti-Fibrogenic Factor Against Intestinal Fibrosis in Crohn's Disease.

Ying Xie, Mieke van Daelen, Rebecca Park, Isabella Almaraz, Lindsey Fontenot, Florian Rieder, Wendy Ho, Berkeley Limketkai, David Q Shih, Minjie Wei and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Ying XieVatche and Tamar Manoukian Division of Digestive Diseases, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA 90095, USA.ORCID 0000-0003-0620-1519
Mieke van DaelenVatche and Tamar Manoukian Division of Digestive Diseases, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA 90095, USA.ORCID 0009-0008-7521-3111
Rebecca ParkVatche and Tamar Manoukian Division of Digestive Diseases, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA 90095, USA.ORCID 0009-0008-5442-3283
Isabella AlmarazVatche and Tamar Manoukian Division of Digestive Diseases, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA 90095, USA.ORCID 0009-0005-9218-5667
Lindsey FontenotVatche and Tamar Manoukian Division of Digestive Diseases, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA 90095, USA.ORCID 0000-0002-7430-5275
Florian RiederDepartment of Gastroenterology, Hepatology, and Nutrition, Digestive Diseases Institute, Department of Inflammation and Immunity, Lerner Research Institute, Program for Global Translational Inflammatory Bowel Diseases, Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, OH 44195, USA.ORCID 0000-0002-9087-1568
Wendy HoVatche and Tamar Manoukian Division of Digestive Diseases, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA 90095, USA.ORCID 0000-0002-7590-4616
Berkeley LimketkaiVatche and Tamar Manoukian Division of Digestive Diseases, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA 90095, USA.ORCID 0000-0003-2714-6934
David Q ShihF. Widjaja Foundation, Inflammatory Bowel & Immunobiology Research Institute, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA.ORCID 0000-0003-1335-7044
Minjie WeiDepartment of Pharmacology, School of Pharmacy, China Medical University, Shenyang 110001, China.ORCID 0000-0001-5548-7280
Yiling LiDepartment of Gastroenterology and Endoscopy, The First Hospital of China Medical University, Shenyang 110001, China.ORCID 0000-0003-3209-8105
Hon Wai KoonVatche and Tamar Manoukian Division of Digestive Diseases, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA 90095, USA.ORCID 0000-0003-0618-0869

Funding

Oral elafin formulation for intestinal fibrosisR01DK128142 · NIDDK · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI KOON, HON WAI · 2022 to 2025
$1.4M
Crohn's and Colitis Foundation 623027NIDDK NIH HHS DK128142NIDDK NIH HHS R01 DK128142
6 · The paper itself

Abstract

More than half of Crohn's disease patients eventually develop intestinal strictures. Intestinal fibrosis is the excessive deposition of the extracellular matrix that obstructs intestinal movement. There is no approved medication to treat intestinal stricture. The roles of serum exosomal miRNAs in intestinal fibrosis are unknown. Serum exosomal miRNA sequencing was performed on samples of healthy donors and stricturing CD (CDS) patients. CDS patient-derived primary intestinal fibroblasts (CD-HIFs), CDS patient-derived serum exosomes (CDSE), human peripheral blood mononuclear cells (PBMCs), human colonic tissues, and mouse models of intestinal fibrosis were used. CDS patients had significantly lower serum exosomal miR205-5p levels than non-CDS patients and healthy donors. CDS patients had reduced miR205-5p expression in PBMCs. miR205-5p reduced its target Zinc finger E-box-binding homeobox 1 (ZEB1) and collagen protein expression in CDSE-treated CD-HIFs. In mouse models of intestinal fibrosis, overexpression of miR205-5p inhibited intestinal fibrosis, which was overcome by Zeb1 overexpression. Elafin, a human anti-fibrogenic protein, induced miR205-5p in intestinal fibroblasts. Inhibition of miR205-5p reversed the anti-fibrogenic effects of elafin in mice. Low serum exosomal anti-fibrogenic miR205-5p levels were associated with intestinal strictures in CD patients. miR205-5p can mediate the anti-fibrogenic effect of elafin by inhibiting ZEB1 and collagen expression.

Indexed as

Crohn DiseaseExosomesIntestinesMicroRNAsAdultAnimalsDisease Models, AnimalFemaleFibroblastsFibrosisHumansMaleMiceZinc Finger E-box-Binding Homeobox 1MicroRNAsZEB1 protein, humanZinc Finger E-box-Binding Homeobox 1exosomefibrosismiRNA

Identifiers

PMID40332426
PMCPMC12028105

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.