ArticleJournal of biomolecular techniques : JBT2024
A Quality Improvement Study of Diagnostic Sural Nerve Morphometry Using Gold Standard Light Microscopy Versus Whole Slide Imaging and Transmission Electron Microscopy.
Article in Journal of biomolecular techniques : JBT, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nerve morphometry is a quantitative diagnostic laboratory technique used to analyze nerve structures, allowing clinicians to detect morphological differences in myelination and assess the progression of peripheral neuropathies. To quantify myelin and axon pathology, a nerve biopsy is prepared for observation using both light microscopy (LM) and transmission electron microscopy (TEM). Standardized morphometry enables the assessment of metrics, including myelinated fiber profile equivalent circle diameter and axon density. This process remains laborious and complex. This quality improvement study aimed to investigate a consolidated approach to conducting nerve morphometry using only TEM, or high-throughput whole-slide imaging (WSI) to replace standard LM imaging, reducing the complexity and time to conduct the procedure. Cases previously biopsied and analyzed for diagnosis by light microscopy were reimaged and assessed utilizing WSI and TEM. Data were then compared to those previously obtained for diagnosis by the standard LM procedure. While the WSI procedure resulted in good sensitivity and specificity (positive and negative predictive values, respectively) compared to the morphometric analyses originally obtained by the standard diagnostic LM, the results from the TEM analysis failed these statistical tests because of the inability to correctly characterize any of the normal cases. Therefore, we conclude that WSI may be an effective alternative to conducting standard LM myelinated fiber morphometry, reducing the time needed to complete the procedure, whereas the TEM morphometry protocol cannot be recommended at this time.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.