Evidence map›Paper›PMID 40330124›Full record

ArticleNpj imaging2025

MRI detection of senescent cells in porcine knee joints with a β-galactosidase responsive Gd-chelate.

Kerem Nernekli, Dilyana B Mangarova, Vidyani Suryadevara, Mohammadjavad Hajipour, Jian-Hong Tang, Jie Wang, Tie Liang, Marek Harris, Tsuyoshi Ueyama, Jennifer K Lyons and 15 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Npj imaging, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Advances in cartilage imaging techniques.Nature reviews. Rheumatology · 2026
    Review
  2. Review
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

25 authors.

Kerem Nernekli *Molecular Imaging Program at Stanford (MIPS), Department of Radiology, Stanford University School of Medicine, Stanford, CA 94305 USA.
Dilyana B Mangarova *Molecular Imaging Program at Stanford (MIPS), Department of Radiology, Stanford University School of Medicine, Stanford, CA 94305 USA.
Vidyani Suryadevara *Molecular Imaging Program at Stanford (MIPS), Department of Radiology, Stanford University School of Medicine, Stanford, CA 94305 USA.
Mohammadjavad HajipourMolecular Imaging Program at Stanford (MIPS), Department of Radiology, Stanford University School of Medicine, Stanford, CA 94305 USA.
Jian-Hong TangDepartment of Chemistry, Molecular Biosciences, Neurobiology and Radiology, Northwestern University, Evanston, IL 60208 USA.
Jie WangMolecular Imaging Program at Stanford (MIPS), Department of Radiology, Stanford University School of Medicine, Stanford, CA 94305 USA.
Tie LiangMolecular Imaging Program at Stanford (MIPS), Department of Radiology, Stanford University School of Medicine, Stanford, CA 94305 USA.
Marek HarrisMolecular Imaging Program at Stanford (MIPS), Department of Radiology, Stanford University School of Medicine, Stanford, CA 94305 USA.
Tsuyoshi UeyamaDivision of Cardiovascular Medicine, Stanford University School of Medicine, Stanford, CA 94305 USA.
Jennifer K LyonsDivision of Cardiovascular Medicine, Stanford University School of Medicine, Stanford, CA 94305 USA.
Michael E MoseleyMolecular Imaging Program at Stanford (MIPS), Department of Radiology, Stanford University School of Medicine, Stanford, CA 94305 USA.
Raheleh RoudiMolecular Imaging Program at Stanford (MIPS), Department of Radiology, Stanford University School of Medicine, Stanford, CA 94305 USA.
Laura PisaniMolecular Imaging Program at Stanford (MIPS), Department of Radiology, Stanford University School of Medicine, Stanford, CA 94305 USA.
Ricarda von KrüchtenMolecular Imaging Program at Stanford (MIPS), Department of Radiology, Stanford University School of Medicine, Stanford, CA 94305 USA.
Ramesh DuwaMolecular Imaging Program at Stanford (MIPS), Department of Radiology, Stanford University School of Medicine, Stanford, CA 94305 USA.
Sarah Ying Lu-LiangMolecular Imaging Program at Stanford (MIPS), Department of Radiology, Stanford University School of Medicine, Stanford, CA 94305 USA.
Zahra Shokri VarniabMolecular Imaging Program at Stanford (MIPS), Department of Radiology, Stanford University School of Medicine, Stanford, CA 94305 USA.
Iryna VasylivMolecular Imaging Program at Stanford (MIPS), Department of Radiology, Stanford University School of Medicine, Stanford, CA 94305 USA.
Neeladrisingha DasDepartment of Radiation Oncology and Medical Physics, Stanford University, Stanford, CA 94305 USA.
Masatoshi MurayamaDepartment of Orthopaedic Surgery, Stanford University, Stanford, CA 94305 USA.
Issei ShinoharaDepartment of Orthopaedic Surgery, Stanford University, Stanford, CA 94305 USA.
Guillem PratxDepartment of Radiation Oncology and Medical Physics, Stanford University, Stanford, CA 94305 USA.
Stuart B GoodmanDepartment of Orthopaedic Surgery, Stanford University, Stanford, CA 94305 USA.
Thomas J MeadeDepartment of Chemistry, Molecular Biosciences, Neurobiology and Radiology, Northwestern University, Evanston, IL 60208 USA.
Heike E Daldrup-LinkMolecular Imaging Program at Stanford (MIPS), Department of Radiology, Stanford University School of Medicine, Stanford, CA 94305 USA.

Funding

Tumor Environment and Metastasis (TEAM) Research ProgramP30CA060553 · NCI · NORTHWESTERN UNIVERSITY AT CHICAGO · PI Devalingam Mahalingam · 1993 to 2026
$153.9M
Translational Oncology Research Program (Project-005)P30CA124435 · NCI · STANFORD UNIVERSITY · PI MICHAEL KENNEY · 2007 to 2026
$71.4M
Monitoring of Stem Cell Engraftment in Arthritic Joints with MR ImagingR01AR054458 · NIAMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI DALDRUP-LINK, HEIKE ELIZABETH · 2008 to 2021
$6.7M
Real-Time Tracking of Gene Therapy by Bioactivated MR contrast ProbesR01NS115571 · NINDS · NORTHWESTERN UNIVERSITY · PI ESTEVES, MIGUEL S, GRAY-EDWARDS, HEATHER L · 2020 to 2024
$3.1M
Cellular Senescence Network: New Imaging Tools for Arthritis ImagingUG3CA268112 · NCI · STANFORD UNIVERSITY · PI DALDRUP-LINK, HEIKE ELIZABETH · 2021 to 2022
$1.1M
NCI NIH HHS P30 CA060553NCI NIH HHS P30 CA124435NCI NIH HHS UG3 CA268112NIAMS NIH HHS R01 AR054458NINDS NIH HHS R01 NS115571
6 · The paper itself

Abstract

Senescent cells promote osteoarthritis progression through the secretion of inflammatory mediators. Preclinical studies have identified senescence-associated beta-galactosidase (β-gal) as a biomarker of senescence, but in vivo detection remains challenging. Here, we evaluated whether a β-gal responsive gadolinium (Gd) chelate can non-invasively detect β-gal expressing senescent cells with standard clinical magnetic resonance imaging (MRI) technology in vitro, ex vivo, and in vivo in porcine joints. In vitro studies showed that senescent mesenchymal stromal cells (MSCs) exhibited significant MRI signal enhancement upon incubation with the β-gal responsive Gd-chelate compared to viable control cells. In vivo, intraarticular injection of the probe into pig knee joints revealed its retention and activation by senescent cells in cartilage defects, evidenced by a significant increase in

Indexed as

Bone imagingMagnetic resonance imagingMolecular imaging

Identifiers

PMID40330124
PMCPMC12049270

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.