Evidence map›Paper›PMID 40330099›Full record

ArticleEcology and evolution2025

Epigenetic Clock in Bears: A Simple Cost-Effective Blood DNA Methylation-Based Age Estimation Method Applicable to Multiple Bear Species.

Michito Shimozuru, Shiori Nakamura, Jumpei Yamazaki, Yojiro Yanagawa, Hiroo Tamatani, Misako Kuroe, Koji Yamazaki, Shinsuke Koike, Yusuke Goto, Tomoko Naganuma and 8 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Ecology and evolution, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors.

Michito ShimozuruFaculty of Veterinary Medicine Hokkaido University Sapporo Hokkaido Japan.ORCID https://orcid.org/0000-0002-3281-8208
Shiori NakamuraFaculty of Veterinary Medicine Hokkaido University Sapporo Hokkaido Japan.
Jumpei YamazakiFaculty of Veterinary Medicine Hokkaido University Sapporo Hokkaido Japan.
Yojiro YanagawaFaculty of Veterinary Medicine Hokkaido University Sapporo Hokkaido Japan.
Hiroo TamataniDepartment of Bear Management Picchio Wildlife Research Center Nagano Japan.
Misako KuroeNagano Environmental Conservation Research Institute Nagano Japan.
Koji YamazakiTokyo University of Agriculture Tokyo Japan.
Shinsuke KoikeTokyo University of Agriculture and Technology Tokyo Japan.
Yusuke GotoZoological Laboratory Ibaraki Nature Museum Ibaraki Japan.
Tomoko NaganumaTokyo University of Agriculture and Technology Tokyo Japan.
Kahoko TochigiTokyo University of Agriculture and Technology Tokyo Japan.
Akino InagakiTokyo University of Agriculture and Technology Tokyo Japan.ORCID https://orcid.org/0000-0002-5724-2815
Naoki TakekoshiTokyo University of Agriculture Tokyo Japan.
Seungyun BaekTokyo University of Agriculture and Technology Tokyo Japan.
Nobutaka SatoAsahikawa City Asahiyama Zoo Asahikawa Hokkaido Japan.
Yusuke HondaNoichi Zoological Park of Kochi Prefecture Konan Kochi Japan.
Toshio TsubotaFaculty of Veterinary Medicine Hokkaido University Sapporo Hokkaido Japan.
Hideyuki ItoWildlife Research Center Kyoto University Kyoto Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Age is an essential factor to understand the life history and demographic parameters of wildlife. Previously, we established an age estimation method for brown bears based on blood DNA methylation level. In this study, we first applied the brown bear-specific age estimation model to other bear species, including Asian black, polar, sun, and Andean bears. Using blood DNA, we performed bisulfite pyrosequencing to determine the methylation levels at four cytosine-phosphate-guanine (CpG) sites adjacent to a single gene,

Indexed as

age estimationagingbearcarnivoreDNA methylationepigenetic clock

Identifiers

PMID40330099
PMCPMC12055220

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.