Utility of green chemistry for spectrofluorometric and spectrophotometric analysis of vericiguat via reaction with erythrocin B.

Food colorant Erythrosine B (EB) is an antiviral xanthene dye with a wide range of uses as a colorant in cosmetics and medications. Its availability, affordability, quick labeling, and high sensitivity make it an excellent choice for spectrofluorometric and spectrophotometric examination of amine-based medications. Two quick and accurate spectrophotometric and spectrofluorometric methods were developed for the estimation of vericiguat in this case. To create an ion-pair complex at pH 4 using the Britton Robinson buffer, the suggested methods relied on the interaction between the amino groups of the medication under study and the phenolic group of EB. The quenching effect of the vericigaute drug of EB at excitation/emission wavelengths of 530.0/550.0 nm. This method demonstrated a limit of detection (LOD) of 0.036 µg/mL and a limit of quantification (LOQ) of 0.110 µg/mL, showing rectilinear response in the concentration range of 0.05-0.5 µg/mL. Additionally, the absorbance of the produced ion-pair complex was evaluated using the colorimetric approach at 560 nm, displaying a linearity range of 0.5-10.0 µg/mL with LOD = 0.428 µg/mL and LOQ = 1.298 µg/mL. The greenness of the developed approaches was determined by GAPT and AGREE software for evaluating the suggested methods.