ArticleGenome medicine2025
Insertion sequences accelerate genomic convergence of multidrug resistance and hypervirulence in Klebsiella pneumoniae via capsular phase variation.
Article in Genome medicine, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Article
- Phenotypic heterogeneity and genetic mechanisms of phage resistance in hypervirulent carbapenem-resistantVirulence · 2026Article
- ElevatedEmerging microbes & infections · 2026Article
- Distribution and Genome Epidemiological Characteristics ofFoods (Basel, Switzerland) · 2026Article
- Genomic Characterisation of Carbapenem-Resistant Klebsiella pneumoniae and Enterobacter hormaechei Clinical Isolates from Nigeria: Evidence of Resistance, Virulence, and Putative Plasmid-Mediated Gene Sharing.Current microbiology · 2026Article
- A rapid molecular assay for the detection of hypervirulentMicrobiology spectrum · 2026Article
- Article
- Genetic control of Group 3 (K96) capsule synthesis and complement resistance in extraintestinal pathogenicmSphere · 2026Article
- Interconnected Reservoirs: Virulence & Biofilm Traits of ESBL-Microorganisms · 2026Article
- Pathogen species and capsule expression shape opportunistic transfer and the recipient response during plasmid RP4 conjugation.Communications biology · 2026Article
- Characterization of Proline-Rich Antimicrobial Peptides with SbmA Transporter-Dependent and Independent Antimicrobial Activity towardACS infectious diseases · 2026Article
- Benefits and costs of a hypercapsule and the mechanism of its loss in a clinical isolate ofmBio · 2026Article
- Exploring the IS-capades ofMicrobial genomics · 2026Article
- Genomic evidence for putative insertion sequence-associated chromosomal integration of aerobactin virulence loci in ST11 carbapenem-resistantFrontiers in microbiology · 2026Article
- Co-evolution of resistance and virulence inFrontiers in cellular and infection microbiology · 2026Review
- Mobile genetic elements in shapingFrontiers in microbiology · 2025Review
- Genomic evolution of enteric pathogens: mechanisms of pathogenicity and diagnostic innovations.Frontiers in microbiology · 2025Review
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10 authors.
Funding
Abstract
backgroundThe convergence of resistance and hypervirulence in Klebsiella pneumoniae represents a significant public health threat, driven by the horizontal transfer of plasmids. Understanding factors affecting plasmid transfer efficiency is essential to elucidate mechanisms behind emergence of these formidable pathogens.
methodsHypermucoviscous K. pneumoniae strains were serially passaged in LB medium to investigate capsule-deficient phenotypes. Capsule-deficient mutants were analyzed using genetic sequencing to identify the types and insertion sites of insertion sequences (IS). Bioinformatics and statistical analyses based on the NCBI and National Microbiology Data Center (NMDC) database were used to map the origins and locations of IS elements. Conjugation assays were performed to assess plasmid transfer efficiency between encapsulated and capsule-deficient strains. A murine intestinal colonization model was employed to evaluate virulence levels and IS excision-mediated capsule restoration.
resultsOur research revealed that a hypervirulent K. pneumoniae (hvKP) strain acquired a bla
conclusionsOur findings reveal that IS elements mediate capsular phase variation by toggling gene activity, accelerating the genomic convergence of multidrug resistance and hypervirulence in K. pneumoniae, as well as facilitating adaptive transitions in different environments.
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