ArticleBMC veterinary research2025
An efficient method for the selective isolation of feline herpesvirus 1(FHV-1) in feline calicivirus (FCV) coinfected specimens.
Article in BMC veterinary research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
2 citing papers in PubMed.
- Review
- Corneal Lesions Associated With Feline Herpesvirus-1 Modified Live Vaccine Strain F2 in Two Cats.Case reports in veterinary medicine · 2026Article
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Authors and funding
12 authors.
Funding
Abstract
backgroundFeline herpesvirus 1 (FHV-1) and feline calicivirus (FCV) are the most common viral pathogens of feline respiratory disease and are highly prevalent in cats worldwide. Coinfection with these viruses is frequent in cats with feline respiratory disease complex (FRDC). It is difficult to isolate pure FHV-1 by conventional laboratory cell culture methods from specimens with FRDC, which brings great trouble to the epidemiological investigation of FHV-1.
methodsFCV polyclonal antibodies were obtained by immunizing rabbits, and the coinfected specimens were neutralized with FCV polyclonal antibodies. Then, virus isolation was performed. After several rounds of neutralization, FHV-1 was finally obtained.
resultsThe FCV polyclonal antibody was successfully obtained with neutralizing activities of 1:128, 1:537, and 1:91. After virus neutralization, the FHV-1 was successfully isolated from the coinfected cell culture suspension and confirmed by immunofluorescence and QRT-PCR.
conclusionIn this study, all FHV-1 present in the coinfection samples were isolated, without any cross-contamination. This method is also theoretically suitable for the isolation and purification of other FCV coinfections or contaminating disease substances.
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