Evidence map›Paper›PMID 40329002›Full record

ArticleGenetica2025

Comparative chloroplast genomic analysis of Pithecellobium dulce (Roxb.) Benth 1844 and related species within Caesalpinioideae.

Hoang Danh Nguyen, Ngoc Han Vu, Hoang Dang Khoa Do, Minh Thiet Vu

Abstract readComparative Study
PubMed Publisher
In one paragraph

Article in Genetica, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Mitochondrial genome dataset ofData in brief · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Hoang Danh NguyenFunctional Genomics Research Center, NTT Hi-Tech Institute, Nguyen Tat Thanh University, Ho Chi Minh City, 70000, Vietnam.ORCID http://orcid.org/0009-0007-0579-2931
Ngoc Han VuFaculty of Integrated Sciences, Fulbright University Vietnam, Saigon Hi-Tech Park, Vo Chi Cong Street, Long Thanh My Ward, Thu Duc City, Ho Chi Minh City, 70000, Vietnam.
Hoang Dang Khoa DoFunctional Genomics Research Center, NTT Hi-Tech Institute, Nguyen Tat Thanh University, Ho Chi Minh City, 70000, Vietnam.ORCID http://orcid.org/0000-0002-7970-9359
Minh Thiet VuFunctional Genomics Research Center, NTT Hi-Tech Institute, Nguyen Tat Thanh University, Ho Chi Minh City, 70000, Vietnam. vmthiet@ntt.edu.vn.ORCID https://orcid.org/0000-0002-7950-0758

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The genus Pithecellobium Mart. (Caesalpinioideae, Fabaceae) plays vital ecological roles, including nitrogen fixation and habitat stabilization, and holds significant medicinal and economic values. However, its genetic diversity and evolutionary relationships remain poorly understood. This study presents the first complete chloroplast (cp.) genome of Pithecellobium dulce (Roxb.) Benth., a widely distributed tropical tree. The complete cp. genome was assembled de novo using NOVOPlasty and annotated with GeSeq and Geneious Prime. Repeat elements and codon usage analyses were analyzed using REPuter, Phobos, and Geneious Prime. Comparative genomic analyses included structural comparisons, IR expansion/contraction, and nucleotide divergence. Phylogenetic relationships were inferred from protein-coding genes using IQ-TREE and MrBayes, with divergence times estimated via BEAST2. The cp. genome of P. dulce was 179,483 bp long, exhibiting a typical quadripartite structure with a large single-copy (LSC) region of 91,513 bp, a small single-copy (SSC) region of 4,560 bp, and two inverted repeat regions (IRs) of 41,705 bp each. It encoded 142 genes, including 97 protein-coding genes, 37 tRNA genes, and eight rRNA genes. Comparative analysis revealed a conserved genomic structure within the Pithecellobium subclade, with notable IR expansion into SSC and LSC regions. Phylogenetic analysis placed P. dulce within the Pithecellobium subclade, closely related to Ebenopsis ebano Britton & Rose and Havardia acatlensis (Benth.) Britton & Rose. Divergence time estimates suggest that Pithecellobium subclade diverged in the late Miocene (~ 29.96 Ma), with P. dulce and E. ebano separating around 16.79 Ma. This study provides an essential genomic resource for resolving phylogenetic relationships and advancing taxonomic research in Caesalpinioideae.

Indexed as

FabaceaeGenome, ChloroplastEvolution, MolecularGenomicsPhylogenyChloroplast genomeFabaceae phylogenomicsIngoid cladeInverted repeat expansionSpecies delimitation

Identifiers

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.