Curtobacterium oceanosedimentum-derived biosurfactants as eco-friendly biocontrol agent with broad-spectrum antibacterial and antifungal properties for sustainable applications.

Amir Mahgoub Awadelkareem, Arif Jamal Siddiqui, Mitesh Patel, Syed Amir Ashraf, Insaf Bahrini, Yazeed Albulaihed, Mohammad Saquib Ashraf, Shahida Banu Shamsuddeen, Mohd Adnan

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Article in World journal of microbiology & biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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5 · Who and what money

Authors and funding

9 authors.

Amir Mahgoub AwadelkareemDepartment of Clinical Nutrition, College of Applied Medical Science, University of Ha'il, P.O. Box 2440, Ha'il, Saudi Arabia.

Arif Jamal SiddiquiDepartment of Biology, College of Science, University of Ha'il, P.O. Box 2440, Ha'il, Saudi Arabia.

Mitesh PatelResearch and Development Cell (RDC), Parul Institute of Applied Sciences, Parul University, Waghodia, Vadodara, Gujarat, 391760, India.

Syed Amir AshrafDepartment of Clinical Nutrition, College of Applied Medical Science, University of Ha'il, P.O. Box 2440, Ha'il, Saudi Arabia.

Insaf BahriniDepartment of Biology, College of Science, University of Ha'il, P.O. Box 2440, Ha'il, Saudi Arabia.

Yazeed AlbulaihedDepartment of Biology, College of Science, University of Ha'il, P.O. Box 2440, Ha'il, Saudi Arabia.

Mohammad Saquib AshrafDepartment of Medical Laboratory Science, College of Pharmacy, Nursing and Medical Sciences, Riyadh ELM University, P.O. Box 12734, Riyadh, Saudi Arabia.

Shahida Banu ShamsuddeenDepartment of Clinical Nutrition, College of Applied Medical Science, University of Ha'il, P.O. Box 2440, Ha'il, Saudi Arabia.

Mohd AdnanDepartment of Biology, College of Science, University of Ha'il, P.O. Box 2440, Ha'il, Saudi Arabia. mo.adnan@uoh.edu.sa.

Funding

University of Hail RG-23208

6 · The paper itself

Abstract

Biosurfactants are eco-friendly surface-active biomolecules with diverse applications in biotechnology, agriculture, and healthcare. This study identifies Curtobacterium oceanosedimentum (C. oceanosedimentum) as a novel biosurfactants producer with potent biocontrol potential against bacterial and fungal pathogens. The present study contributes to ongoing efforts in biosurfactant research by presenting the functional attributes of C. oceanosedimentum-derived biosurfactants (COBS), characterized as a complex glycolipopeptide or lipoglycoprotein containing lipid, peptide, and carbohydrate moieties. Preliminary screening through oil spreading, drop collapse, and hemolytic assays confirmed biosurfactants production, while emulsification index assays demonstrated strong emulsifying activity, particularly with olive oil (60%), indicating broad applicability. Advanced characterization using HPTLC, FTIR, and HR-LCMS revealed the presence of amino acids, peptides, and fatty acid amides, further validating its structural complexity. Antimicrobial assays demonstrated superior efficacy of COBS, exhibiting significant antibacterial activity against Chromobacterium violaceum (24 mm) and Acinetobacter calcoaceticus (22 mm), alongside pronounced antifungal effects against Candida albicans, Fusarium proliferatum, and Penicillium purpurogenum. These findings establish COBS as a unique biosurfactants with dual antibacterial and antifungal properties, distinguishing it from previously reported biosurfactants. Its eco-friendly nature and broad-spectrum antimicrobial potential make it a promising candidate for sustainable applications in biocontrol, healthcare, and environmental remediation. Further studies on large-scale production and stability optimization will be essential for commercial applications.

Indexed as

Anti-Bacterial AgentsAntifungal AgentsBiological Control AgentsSurface-Active AgentsBacteriaBiosurfactantsChromobacteriumFungiMicrobial Sensitivity TestsAnti-Bacterial AgentsAntifungal AgentsBiological Control AgentsSurface-Active AgentsAntibacterial activityAntifungal activityBiocontrol agentBioremediationBiosurfactantsCurtobacterium oceanosedimentumSustainable applications

Identifiers

PMID40328999

DOI10.1007/s11274-025-04382-y

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.