ArticleApplied microbiology and biotechnology2025
Influence of orthodontic appliances and nitrate on the oral microbiota.
Article in Applied microbiology and biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed.
- Role of oral and gut microbiomes in enterosalivary nitrate metabolism and their effects on systemic disease.Frontiers in cellular and infection microbiology · 2025Review
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this pilot study, we investigated the bacterial changes introduced on the subgingival, tongue, and saliva microbiota during fixed orthodontic treatment, with or without daily administration of nitrate-containing beet juice for 2 weeks in 22 individuals with good general health. We followed clinical parameters in combination with microbiota changes before, after 2 weeks, and after 6 months of treatment with fixed orthodontic appliances. In accordance with variations in community composition at the sampling sites, effects to orthodontic treatment differed. Subgingival communities responded promptly to orthodontic treatment with no additional structural changes over time, whereas saliva and tongue communities were affected only after extended treatment. Periodontal pathogens such as Selenomonas sputigena were enriched in subgingival communities, whereas Streptococcus mutans was enriched in saliva. Specifically, Rothia mucilaginosa increased tremendously in relative abundance in both tongue and saliva communities. The effect of beet juice on microbial composition was significant in subgingival samples even though the differences were not mirrored in single differentially distributed genera or species. This indicates changes in the complete subgingival microbial net of interacting species. However, the prevention of Corynebacterium matruchotii enrichment by beet juice may be important for prevention of biofilm formation. Enrichment of Neisseria flavescens group bacteria and Abiotrophia and depletion of different Actinomyces and Stomatobaculum were observed on tongue communities. We conclude that subgingival microbiota are rapidly affected by fixed orthodontic appliances and can be positively influenced by regular administration of nitrate-containing juice. KEY POINTS: • The subgingival site, tongue, and saliva contain different microbiota • The microbiota react differently to orthodontic treatment and beet juice • Key genera and species affected by treatments were identified.
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