Evidence map›Paper›PMID 40328889›Full record

ArticleScientific reports2025

Introns increase gene expression in Caenorhabditis elegans by a mechanism that must be at least partly different than in plants.

Alan B Rose, Aaron Baer, Isaac Shaker, J Grey Monroe, Ian Korf, Lesilee S Rose

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Alan B RoseDepartment of Molecular and Cellular Biology, University of California, Davis, 95616, USA. abrose@ucdavis.edu.
Aaron BaerDepartment of Molecular and Cellular Biology, University of California, Davis, 95616, USA.
Isaac ShakerDepartment of Molecular and Cellular Biology, University of California, Davis, 95616, USA.
J Grey MonroeDepartment of Plant Sciences, University of California, Davis, 95616, USA.
Ian KorfDepartment of Molecular and Cellular Biology, University of California, Davis, 95616, USA.
Lesilee S RoseDepartment of Molecular and Cellular Biology, University of California, Davis, 95616, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The wide diversity of organisms in which introns stimulate gene expression suggests that this is an ancient phenomenon. However, the mechanisms through which introns boost expression remain poorly understood, and the degree the which the action of introns is evolutionarily conserved is unknown. Here we compared the effect on expression of introns at different positions and tested ten different introns at the same location in a reporter gene in single-copy transgenic nematodes. The introns boosted expression most when near the start of the gene, as previously observed in several organisms. All ten introns tested at the same position increased mRNA accumulation 10- to 17-fold, in contrast to plants where introns vary widely in their effect on expression and relatively few increase mRNA levels 10-fold or more. These results suggest that some aspects of the mechanisms through which introns boost expression are fundamentally different in nematodes and plants.

Indexed as

Caenorhabditis elegansGene Expression RegulationIntronsPlantsAnimalsAnimals, Genetically ModifiedGenes, ReporterRNA, MessengerRNA, MessengerGene expressionIntron-mediated enhancementIntronsmRNA accumulationNematode

Identifiers

PMID40328889
PMCPMC12055998

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.