Evidence map›Paper›PMID 40328789›Full record

ArticleScientific reports2025

TNF induces catabolism in human cartilaginous endplate cells in 3D agarose culture under dynamic compression.

Katherine B Crump, Exarchos Kanelis, Maria Segarra-Queralt, Andreu Pascuet-Fontanet, Paola Bermudez-Lekerika, Ahmad Alminnawi, Liesbet Geris, Leonidas G Alexopoulos, Jérôme Noailly, Benjamin Gantenbein

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Katherine B CrumpTissue Engineering for Orthopaedics & Mechanobiology, Bone & Joint Program, Department for BioMedical Research (DBMR), Faculty of Medicine, University of Bern, 3008, Bern, Switzerland.ORCID http://orcid.org/0000-0001-5328-667X
Exarchos KanelisSchool of Mechanical Engineering, National Technical University of Athens, 15772, Zografou, Greece.ORCID http://orcid.org/0000-0002-2059-1480
Maria Segarra-QueraltBCN Medtech, Universitat Pompeu Fabra, 08018, Barcelona, Spain.ORCID http://orcid.org/0000-0001-9332-0764
Andreu Pascuet-FontanetBCN Medtech, Universitat Pompeu Fabra, 08018, Barcelona, Spain.ORCID http://orcid.org/0000-0003-3610-877X
Paola Bermudez-LekerikaTissue Engineering for Orthopaedics & Mechanobiology, Bone & Joint Program, Department for BioMedical Research (DBMR), Faculty of Medicine, University of Bern, 3008, Bern, Switzerland.ORCID http://orcid.org/0000-0002-6858-8213
Ahmad AlminnawiGIGA In Silico Medicine, University of Liège, Liège, 4000, Belgium.ORCID http://orcid.org/0000-0002-9998-3275
Liesbet GerisGIGA In Silico Medicine, University of Liège, Liège, 4000, Belgium.ORCID http://orcid.org/0000-0002-8180-1445
Leonidas G AlexopoulosSchool of Mechanical Engineering, National Technical University of Athens, 15772, Zografou, Greece.ORCID http://orcid.org/0000-0003-0425-166X
Jérôme NoaillyBCN Medtech, Universitat Pompeu Fabra, 08018, Barcelona, Spain.ORCID http://orcid.org/0000-0003-3446-7621
Benjamin GantenbeinTissue Engineering for Orthopaedics & Mechanobiology, Bone & Joint Program, Department for BioMedical Research (DBMR), Faculty of Medicine, University of Bern, 3008, Bern, Switzerland. Benjamin.Gantenbein@unibe.ch.ORCID http://orcid.org/0000-0002-9005-0655

Funding

H2020 Marie Skłodowska-Curie Actions #955735
6 · The paper itself

Abstract

Intervertebral disc (IVD) degeneration is the leading cause of low back pain in young adults, and the cartilaginous endplate (CEP) is likely to play a key role in early IVD degeneration. To elucidate the effects of pro-inflammatory cytokines on the mechanobiology of the CEP, human CEP cells were seeded into 2% agarose, dynamically compressed up to 7%, and stimulated with tumor necrosis factor (TNF). It was hypothesized that dynamic compression would be sufficient to induce anabolism, while stimulation with TNF would induce catabolism. TNF was sufficient to induce a catabolic, time-dependent response in human CEP cells through downregulation of anabolic gene expression and increased secretion of pro-inflammatory proteins associated with herniated discs, bacteria inhibition, and pain. However, 7% strain or scaffold material, agarose, may not lead to full activation of integrins and downregulation of pro-inflammatory pathways, demonstrated in part through the unchanged gene expression of integrin subunits α

Indexed as

CartilageIntervertebral DiscTumor Necrosis Factor-alphaCells, CulturedHumansIntervertebral Disc DegenerationSepharoseSepharoseTumor Necrosis Factor-alphaAgaroseCartilaginous endplate 3D cultureCatabolismDynamic compressionHydrogelIntervertebral discMechanobiologyPro-inflammatory cytokines

Identifiers

PMID40328789
PMCPMC12056083

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.