Evidence map›Paper›PMID 40327128›Full record

ArticleApplied microbiology and biotechnology2025

Indirect ELISA developed to detect antibodies against Mycoplasma synoviae P50 protein via immunoproteomic screening.

Yang Liu, Guangju You, Jialei Shi, Li Gao, Xiaoqi Li, Hong Cao, Yongqiang Wang, Shijun J Zheng

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Applied microbiology and biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Yang LiuCollege of Veterinary Medicine, National Key Laboratory of Veterinary Public Health Security, China Agricultural University, Beijing, China.
Guangju YouCollege of Veterinary Medicine, National Key Laboratory of Veterinary Public Health Security, China Agricultural University, Beijing, China.
Jialei ShiCollege of Veterinary Medicine, National Key Laboratory of Veterinary Public Health Security, China Agricultural University, Beijing, China.
Li GaoCollege of Veterinary Medicine, National Key Laboratory of Veterinary Public Health Security, China Agricultural University, Beijing, China.
Xiaoqi LiCollege of Veterinary Medicine, National Key Laboratory of Veterinary Public Health Security, China Agricultural University, Beijing, China.
Hong CaoCollege of Veterinary Medicine, National Key Laboratory of Veterinary Public Health Security, China Agricultural University, Beijing, China.
Yongqiang WangCollege of Veterinary Medicine, National Key Laboratory of Veterinary Public Health Security, China Agricultural University, Beijing, China. vetwyq@cau.edu.cn.
Shijun J ZhengCollege of Veterinary Medicine, National Key Laboratory of Veterinary Public Health Security, China Agricultural University, Beijing, China. sjzheng@cau.edu.cn.

Funding

the National Key Research and Development Program of China grant number 2022YFD 1300100
6 · The paper itself

Abstract

Mycoplasma synoviae infection is a chronic disease of poultry with significant economic impacts. An efficient diagnostic tool for M. synoviae infection is in great demand. This study aimed to develop a novel indirect enzyme-linked immunosorbent assay (iELISA) method based on antigens identified by pull-down assay combined with mass spectrometry. Using these methods and anti-M. synoviae serum, we identified an uncharacterized protein with a molecular weight of 53 kDa (named P50 protein) and then established a recombinant P50 protein-based ELISA (rP50-ELISA) to detect antibodies against P50 protein. A receiver operating characteristic (ROC) analysis was performed to estimate the optical density (OD) cut-off value that maximized the sensitivity (Se) and specificity (Sp) of the rP50-ELISA, which had a mean Se of 93% (95% confidence interval (CI) = 86.25-96.57%) and a mean Sp of 100% (95% CI = 91.80-100%), with an area under the curve (AUC) of 0.9979 (95% CI = 99.41-100%). The rP50-ELISA showed no cross-reactivity with antibodies against other avian pathogens. Serum samples from 164 clinical chickens were tested with the rP50-ELISA, and the results revealed a high concordance rate of 93.29% with commercial diagnostic kits. KEY POINTS: • Screening for the major antigen of M. synoviae for ELISA development. • The P50 protein was selected as a coating antigen for ELISA. • rP50-ELISA was successfully developed for detecting anti-M. synoviae antibodies with high sensitivity and specificity.

Indexed as

Antibodies, BacterialBacterial ProteinsMycoplasma InfectionsMycoplasma synoviaePoultry DiseasesAnimalsAntigens, BacterialChickensEnzyme-Linked Immunosorbent AssayProteomicsROC CurveSensitivity and SpecificityAntibodies, BacterialAntigens, BacterialBacterial ProteinsIndirect ELISALipoproteinMycoplasma synoviaeP50 protein

Identifiers

PMID40327128
PMCPMC12055914

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.