Evidence map›Paper›PMID 40326462›Full record

ArticleJournal of cell science2025

Quantitative comparison of fluorescent proteins using protein nanocages in live cells.

Giulia Viola, Yasmeen W Ibrahim, Kyle A Jacobs, Joël Lemière, Matthew L Kutys, Torsten Wittmann

Abstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of cell science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Giulia ViolaDepartment of Cell and Tissue Biology, University of California, San Francisco, San Francisco, CA 94143, USA.ORCID 0009-0000-6056-0411
Yasmeen W IbrahimDepartment of Cell and Tissue Biology, University of California, San Francisco, San Francisco, CA 94143, USA.ORCID 0009-0003-5959-0068
Kyle A JacobsDepartment of Cell and Tissue Biology, University of California, San Francisco, San Francisco, CA 94143, USA.ORCID 0000-0002-4411-7855
Joël LemièreDepartment of Cell and Tissue Biology, University of California, San Francisco, San Francisco, CA 94143, USA.ORCID 0000-0002-9017-1959
Matthew L KutysDepartment of Cell and Tissue Biology, University of California, San Francisco, San Francisco, CA 94143, USA.ORCID 0000-0002-0752-649X
Torsten WittmannDepartment of Cell and Tissue Biology, University of California, San Francisco, San Francisco, CA 94143, USA.ORCID 0000-0001-9134-691X

Funding

Decoding cortical Notch signaling and morphogenic instruction at cell-cell interfacesR35GM150987 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI KUTYS, MATTHEW L · 2023 to 2025
$1.3M
Quantitative analysis and manipulation of chromosome dynamics in cancer organoidsR61CA278518 · NCI · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI Torsten Wittmann · 2024 to 2026
$680k
Wide-field super-resolution spinning disk confocal microscopeS10OD028611 · OD · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI WITTMANN, TORSTEN · 2020 to 2020
$600k
Spinning disk confocal / FRAP microscope for quantitative live cell imagingS10RR026758 · NCRR · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI WITTMANN, TORSTEN · 2010 to 2010
$500k
National Science Foundation 2038436NCI NIH HHS R61 CA278518NCRR NIH HHS S10 RR026758NIGMS NIH HHS R35 GM150987NIH HHS R61 CA278518NIH HHS S10 OD028611NIH HHS S10 RR026758ODCDC CDC HHS S10 OD028611Tobacco-Related Disease Research Program T33DT6442University of California
6 · The paper itself

Abstract

To standardize comparison of fluorescent protein performance on a molecule-by-molecule basis in a physiological intracellular environment, we constructed fluorescent protein-tagged I3-01 peptides that self-assemble into stable 60-subunit dodecahedrons inside live mammalian cells. We were especially interested in determining which of the recently published monomeric StayGold variants is best for live microscopy in mammalian cells. Combining nanocage brightness and photobleaching measurements into a single metric, mStayGold stood out as far superior to all other green and red fluorescent proteins we tested with a functional lifetime that is at least 8-10-fold longer compared with EGFP or mEmerald. Analysis of intracellular nanocage diffusion further confirmed the monomeric nature of mStayGold, and we demonstrate that mStayGold-tagged nanocages can serve as highly photostable nanoparticles to analyze intracellular biophysical properties. Analysis of frequently used red fluorescent proteins was less encouraging and recent mScarlet or mRuby variants did not perform substantially better than mCherry on a typical spinning disc confocal microscope system, highlighting the importance of a standardized method to benchmark fluorescent proteins to make optimal choices for specific experimental setups.

Indexed as

Luminescent ProteinsAnimalsGreen Fluorescent ProteinsHumansPhotobleachingRed Fluorescent ProteinGreen Fluorescent ProteinsLuminescent ProteinsRed Fluorescent ProteinFluorescent protein comparisonIntracellular diffusionLive-cell fluorescence microscopymScarletmStayGoldProtein nanocages

Identifiers

PMID40326462
PMCPMC12148025

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.