Evidence map›Paper›PMID 40325428›Full record

ReviewJournal of medical case reports2025

The application of metagenomic next generation sequencing in diagnosing tuberculous otitis media: a case report and review of the literature.

HongYan Liu, YiFan Zhu, YangYiYi Huang, Hua Jiang

Abstract readCase ReportsReview
PubMed Full text at PMC Publisher
In one paragraph

Review in Journal of medical case reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

HongYan LiuDepartment of Otolaryngology, The Second Affiliated Hospital Zhejiang University School of Medicine, Hangzhou, China.
YiFan ZhuDepartment of Otolaryngology, The Second Affiliated Hospital Zhejiang University School of Medicine, Hangzhou, China.
YangYiYi HuangDepartment of Otolaryngology, The Second Affiliated Hospital Zhejiang University School of Medicine, Hangzhou, China.
Hua JiangDepartment of Otolaryngology, The Second Affiliated Hospital Zhejiang University School of Medicine, Hangzhou, China. zrjh@zju.edu.cn.

Funding

Key Research and Development Program of Zhejiang Province 2024C03238Natural Science Foundation of Zhejiang Province LQ24H130002
6 · The paper itself

Abstract

backgroundTuberculous otitis media is a chronic Mycobacterium tuberculosis infection of the middle ear tissues. Diseases with varied and insidious clinical features can make diagnosis difficult and delay treatment. CASE PRESENTATION: Here, we document a case of tuberculous otitis media in a 46-year-old ethnic Han woman that manifested as nonspecific chronic otitis media. A mastoidectomy and tympanoplasty were performed for the initial diagnosis of cholesteatoma. The histopathology of the tissue specimen revealed granuloma formation with necrosis. Staining for acid-fast bacilli and the polymerase chain reaction method for Mycobacterium tuberculosis yielded negative results. However, the chest computed tomography scan demonstrated a pulmonary miliary nodule. Next, metagenomic next-generation sequencing was applied and the Mycobacterium tuberculosis was identified. The patient recovered after receiving antituberculous treatment.

conclusionThis report highlights the application of novel diagnostic tools such as metagenomic next-generation sequencing as a supplementary method for the diagnosis of tuberculous otitis media in highly suspected patients.

Indexed as

High-Throughput Nucleotide SequencingMetagenomicsMycobacterium tuberculosisOtitis MediaTuberculosisAntitubercular AgentsFemaleHumansMastoidectomyMiddle AgedTomography, X-Ray ComputedAntitubercular AgentsCase reportDiagnosisMetagenomic next-generation sequencingOtitis mediaTuberculous

Identifiers

PMID40325428
PMCPMC12051264

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.