ArticleJournal of virology1985
Expression of Sindbis virus structural proteins via recombinant vaccinia virus: synthesis, processing, and incorporation into mature Sindbis virions.
Article in Journal of virology, 1985. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 90 citations in OpenAlex.
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- A fold switch regulates conformation of an alphavirus virus RNA-dependent RNA polymerase.bioRxiv : the preprint server for biology · 2025Article
- Genomic sequence and virulence of clonal isolates of vaccinia virus Tiantan, the Chinese smallpox vaccine strain.PloS one · 2013Article
- An attenuated LC16m8 smallpox vaccine: analysis of full-genome sequence and induction of immune protection.Journal of virology · 2005Article
- Immunization with nonstructural proteins promotes functional recovery of alphavirus-infected neurons.Journal of virology · 1997Article
- Effect of serum type and concentration on the expression ofβ-galactosidase in recombinant vaccinia virus infected HeLa S3 cells.Cytotechnology · 1995Article
- The alphaviruses: gene expression, replication, and evolution.Microbiological reviews · 1994Review
- Cleavage at a novel site in the NS4A region by the yellow fever virus NS2B-3 proteinase is a prerequisite for processing at the downstream 4A/4B signalase site.Journal of virology · 1993Article
- Trypanosoma cruzi flagellar repetitive antigen expression by recombinant baculovirus: towards an improved diagnostic reagent for Chagas' disease.Bio/technology (Nature Publishing Company) · 1992Article
- Rescue of Sindbis virus-specific RNA replication and transcription by using a vaccinia virus recombinant.Journal of virology · 1991Article
- Mutagenesis of the putative fusion domain of the Semliki Forest virus spike protein.Journal of virology · 1991Article
- Assembly and analysis of a functional vaccinia virus "amplicon" containing the C-repeat region from the M protein of Streptococcus pyogenes.Proceedings of the National Academy of Sciences of the United States of America · 1991Article
- Drosophila stimulatory G protein alpha subunit activates mammalian adenylyl cyclase but interacts poorly with mammalian receptors: implications for receptor-G protein interaction.Proceedings of the National Academy of Sciences of the United States of America · 1991Article
- Structural proteins of hog cholera virus expressed by vaccinia virus: further characterization and induction of protective immunity.Journal of virology · 1991Article
- Expression of the NS1 gene of dengue virus type 2 using vaccinia virus. Dimerisation of the NS1 glycoprotein.Archives of virology · 1991Article
- Vaccinia virus vectors: new strategies for producing recombinant vaccines.Clinical microbiology reviews · 1990Review
- Fusion function of the Semliki Forest virus spike is activated by proteolytic cleavage of the envelope glycoprotein precursor p62.Journal of virology · 1990Article
- Expression of the structural proteins of dengue 2 virus and yellow fever virus by recombinant vaccinia viruses.Archives of virology · 1990Article
- Nucleotide sequence and transcriptional studies of the vaccinia virus KpnI I DNA fragment.Virus genes · 1989Article
- Expression of Drosophila Shaker potassium channels in mammalian cells infected with recombinant vaccinia virus.Proceedings of the National Academy of Sciences of the United States of America · 1989Article
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Authors and funding
4 authors.
Funding
Abstract
We have obtained a vaccinia virus recombinant which contains a complete cDNA copy of the 26S RNA of Sindbis virus within the thymidine kinase gene of the vaccinia virus genome. This recombinant constitutively transcribed the Sindbis sequences throughout the infectious cycle, reflecting the dual early-late vaccinia promoter used in this construction. The Sindbis-derived transcripts were translationally active, giving rise to both precursor and mature structural proteins of Sindbis virus, including the capsid protein (C), the precursor of glycoprotein E2 (PE2), and the two mature envelope glycoproteins (E1 and E2). These are the same products translated from the 26S mRNA during Sindbis infection, and thus these proteins were apparently cleaved, glycosylated, and transported in a manner analogous to that seen during authentic Sindbis infections. By using epitope-specific antibodies, it was possible to demonstrate that recombinant-derived proteins were incorporated into Sindbis virions during coinfections with monoclonal antibody-resistant Sindbis variants. These results suggest that all the information necessary to specify the proper biogenesis of Sindbis virus structural proteins resides within the 26S sequences and that vaccinia may provide an appropriate system for using DNA molecular genetic manipulations to unravel a variety of questions pertinent to RNA virus replication.
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