Evidence map›Paper›PMID 40325125›Full record

ArticleScientific reports2025

Production of live tiger salamander offspring using cryopreserved sperm.

Devin M Chen, Shaina S Lampert, Li-Dunn Chen, Isabella J Burger, Carrie K Kouba, Peter J Allen, Nucharin Songsasen, Terri L Roth, Andrew J Kouba

Abstract read
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In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Devin M ChenDepartment of Wildlife, Fisheries, and Aquaculture, Mississippi State University, Thompson Hall, 775 Stone Boulevard, Mississippi State, MS, 39762, USA.
Shaina S LampertDepartment of Biochemistry, Molecular Biology, Entomology, and Plant Pathology, Mississippi State University, 402 Dorman Hall, Mississippi State, MS, 39762, USA.
Li-Dunn ChenDepartment of Biochemistry, Molecular Biology, Entomology, and Plant Pathology, Mississippi State University, 402 Dorman Hall, Mississippi State, MS, 39762, USA.
Isabella J BurgerDepartment of Wildlife, Fisheries, and Aquaculture, Mississippi State University, Thompson Hall, 775 Stone Boulevard, Mississippi State, MS, 39762, USA.
Carrie K KoubaDepartment of Biochemistry, Molecular Biology, Entomology, and Plant Pathology, Mississippi State University, 402 Dorman Hall, Mississippi State, MS, 39762, USA.
Peter J AllenDepartment of Wildlife, Fisheries, and Aquaculture, Mississippi State University, Thompson Hall, 775 Stone Boulevard, Mississippi State, MS, 39762, USA.
Nucharin SongsasenCenter for Species Survival, Smithsonian National Zoo and Conservation Biology Institute, 1500 Remount Road, Front Royal, VA, 22630, USA.
Terri L RothCenter for Conservation and Research of Endangered Wildlife, Cincinnati Zoo and Botanical Garden, 3400 Vine Street, Cincinnati, OH, 45220, USA.
Andrew J KoubaDepartment of Wildlife, Fisheries, and Aquaculture, Mississippi State University, Thompson Hall, 775 Stone Boulevard, Mississippi State, MS, 39762, USA. a.kouba@msstate.edu.

Funding

Agricultural Research Service 6066-31000- 015-00DInstitute of Museum and Library Services MG-30-17-0052-17National Institute of Food and Agriculture W3173
6 · The paper itself

Abstract

Amphibian conservation breeding programs often rely on assisted reproductive technologies (ART) to produce offspring due to the difficulty of replicating natural reproductive cues under human care. Reproductive management using in-vitro fertilization (IVF) with cryopreserved sperm has been successfully applied to a variety of anurans, yet the approach has seen limited use for internally-fertilizing caudates. This study aimed to test two cryoprotectants, dimethyl formamide (DMFA) and dimethyl sulfoxide (DMSO), in combination with three different sperm extenders, HAM's F-10, diluted HAM's F-10, and 10% Holtfreter's, for the production of salamander larvae from cryopreserved sperm. Fresh sperm was collected from tiger salamanders (n = 12) and diluted with one of three extenders, then subsequently mixed 1:1 with cryoprotectant. Samples were frozen using liquid nitrogen and later thawed to be applied in IVF. The diluted HAM's and DMFA treatment resulted in the highest (p < 0.05) fertilization rate, with over 40% of eggs cleaving. The DMFA treatments resulted in 87 hatched offspring, while DMSO treatments resulted in only one offspring. This research highlights the potential of biobanking to produce internally-fertilizing salamander larvae, with DMFA being the likely preferred sperm cryoprotectant. These findings can inform conservation breeding programs by increasing caudate population sustainability through genetic management, facilitated by ART.

Indexed as

AmbystomaCryopreservationSemen PreservationSpermatozoaAnimalsCryoprotective AgentsDimethylformamideDimethyl SulfoxideFemaleFertilization in VitroLarvaMaleCryoprotective AgentsDimethylformamideDimethyl SulfoxideAmphibianAssisted reproductionBreedingConservationCryosolutionEx-situ

Identifiers

PMID40325125
PMCPMC12053565

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.