Evidence map›Paper›PMID 40323490›Full record

ArticleBiotechnology letters2025

Construction of Corynebacterium glutamicum chassis for preferential utilization of xylose by adaptive laboratory evolution.

Mingxin Gao, Xi Sun, Yiyang Liu, Tao Chen, Zhiwen Wang

Abstract read
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In one paragraph

Article in Biotechnology letters, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Mingxin Gao *State Key Laboratory of Synthetic Biology, Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China.
Xi Sun *School of Life Sciences, Ningxia University, Yinchuan, 750021, China.
Yiyang LiuState Key Laboratory of Synthetic Biology, Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China.
Tao ChenState Key Laboratory of Synthetic Biology, Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China.
Zhiwen WangState Key Laboratory of Synthetic Biology, Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China. zww@tju.edu.cn.ORCID http://orcid.org/0000-0001-8938-3631

Funding

Central Guidance for Local Science and Technology Development Projects 2024FRD05057National Natural Science Foundation of China NSFC-22278312Ningxia Hui Autonomous Region 2024BEE02005
6 · The paper itself

Abstract

objectivesTo fully utilize lignocellulosic hydrolysate abundant in xylose content, it is necessary to engineer a Corynebacterium glutamicum strain that can preferentially and efficiently utilize xylose in the presence of glucose/xylose mixtures.

resultsC. glutamicum strain CGS15X5-E2 was obtained through metabolic engineering and adaptive laboratory evolution (ALE), which preferentially utilizes xylose and completely consumes it before switching to glucose utilization. A genetically defined chassis strain, CGS15X57, was constructed to switch to glucose consumption only after xylose was depleted based on genome analysis and mutation reconstruction, in which xylose utilization capability was also enhanced. The average xylose consumption rate of CGS15X57 reached 0.833 ± 0.048 g/l/h, which was 28.0% higher than that of the control. Three new beneficial mutations (Cgl1992

conclusionsA chassis strain of C. glutamicum that preferentially and efficiently utilizes xylose has been obtained, facilitating the full utilization of lignocellulosic hydrolysates and the construction of co-culture systems under glucose/xylose mixed sugar conditions.

Indexed as

Corynebacterium glutamicumMetabolic EngineeringXyloseDirected Molecular EvolutionGlucoseGlucoseXyloseCarbon catabolite repressionCorynebacterium glutamicumLignocellulose hydrolysateModular co-culture strategyXylose

Identifiers

PMID40323490

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.