Application of lactose based autoinduction for heterologous production of an active [NiFe] hydrogenase in E. coli.

Francisco de la Fuente-Kratzborn, Qin Fan, Peter Neubauer, Matthias Gimpel

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Article in Biotechnology letters, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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Authors and funding

4 authors.

Francisco de la Fuente-KratzbornChair of Bioprocess Engineering, Institute of Biotechnology, Technische Universität Berlin, Ackerstr. 76, ACK24, D‑13355, Berlin, Germany.

Qin FanChair of Bioprocess Engineering, Institute of Biotechnology, Technische Universität Berlin, Ackerstr. 76, ACK24, D‑13355, Berlin, Germany. qin.fan@tu-berlin.de.ORCID http://orcid.org/0000-0001-5801-8690

Peter NeubauerChair of Bioprocess Engineering, Institute of Biotechnology, Technische Universität Berlin, Ackerstr. 76, ACK24, D‑13355, Berlin, Germany.

Matthias GimpelChair of Bioprocess Engineering, Institute of Biotechnology, Technische Universität Berlin, Ackerstr. 76, ACK24, D‑13355, Berlin, Germany.

Funding

Deutsche Forschungsgemeinschaft 2008/1-390540038

6 · The paper itself

Abstract

objectivesThis study aims to assess whether a fed-batch-based auto-induction method can enhance active hydrogenase production, encompassing cofactor formation and space-time yield.

resultsThe recombinant Escherichia coli strain BQF8RH8, possessing plasmids for assembly and proper maturation of Cupriavidus necator regulatory hydrogenase (RH), was cultivated in the fed-batch like EnPresso B medium with an autoinduction lactose-based system. In contrast to classical IPTG induction previously performed, we obtained active RH demonstrating the feasibility of the process for active hydrogenase production.

conclusionOur results affirm the viability of the previously developed auto-induction strategy also for a functional hydrogenase with the complex maturation process. This significantly accelerates the process and enhances hydrogenase productivity.

Indexed as

Escherichia coliHydrogenaseLactoseBatch Cell Culture TechniquesCulture MediaCupriavidus necatorRecombinant ProteinsCulture MediaHydrogenaseLactosenickel-iron hydrogenaseRecombinant ProteinsAutoinductionCupriavidus necator[NiFe]-hydrogenaseRegulatory hydrogenase

Identifiers

PMID40323466

DOI10.1007/s10529-025-03594-4

PMCPMC12053349

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