Evidence map›Paper›PMID 40323208›Full record

ArticlePhysiological reports2025

Void spot assay visualization optimization for use of Void Whizzard in rats (Rattus norvegicus).

Hannah Ruetten, Jadyn Bothe, Shannon Lankford, Gopal Badlani, James Koudy Williams

Abstract read
In one paragraph

Article in Physiological reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

5 authors.

Hannah RuettenWake Forest Institute for Regenerative Medicine, Wake Forest Baptist Medical Center, Winston-Salem, North Carolina, USA.ORCID 0000-0001-6263-6221
Jadyn BotheWake Forest Institute for Regenerative Medicine, Wake Forest Baptist Medical Center, Winston-Salem, North Carolina, USA.
Shannon LankfordWake Forest Institute for Regenerative Medicine, Wake Forest Baptist Medical Center, Winston-Salem, North Carolina, USA.
Gopal BadlaniDepartment of Urology, Wake Forest Baptist Medical Center Winston-Salem, Winston-Salem, North Carolina, USA.
James Koudy WilliamsWake Forest Institute for Regenerative Medicine, Wake Forest Baptist Medical Center, Winston-Salem, North Carolina, USA.

Funding

Laboratory Animal & Comparative Medicine TrainingT32OD010957 · OD · WAKE FOREST UNIVERSITY HEALTH SCIENCES · PI J. MARK CLINE · 2012 to 2026
$4.3M
Tissue Engineering and Regenerative Medicine Undergraduate Research Addressing Challenges In Diabetes, Kidney, Urologic and Hematologic DiseasesR25DK121572 · NIDDK · WAKE FOREST UNIVERSITY HEALTH SCIENCES · PI OPARA, EMMANUEL C. · 2019 to 2019
$99k
HHS | NIH | National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) NIH R25 DK121572HHS | NIH | NIH Office of the Director (OD) NIH T32 OD010957NIDDK NIH HHS R25 DK121572NIH HHS T32 OD010957the U.S. Department of Veterans Affairs
6 · The paper itself

Abstract

Void spot assay (VSA) noninvasively evaluates urination. This study optimizes VSA by comparing post-assay paper visualization techniques: bright field light (BF), ultraviolet light (UV), and ninhydrin spray (N). Male rats were placed in filter paper lined cages for 4 h. After the assay, all papers were dried. BF images were photographed (digital camera). UV images were captured using a Darkroom ultraviolet imaging cabinet. Papers were sprayed with ninhydrin and photographed (digital camera). All images were converted to binary for analysis with Void Whizzard. UV versus BF significantly differed in area. All three groups significantly differed in overall spot count and spots 0-0.1 cm

Indexed as

UrinationAnimalsMaleNinhydrinRatsRats, Sprague-DawleyUltraviolet RaysNinhydrinlower urinary tract dysfunctionmicturitionninhydrinurinary dysfunctionurine

Identifiers

PMID40323208
PMCPMC12051384

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.